ArticleBMC genomics2015
Quantitative proteomic analysis of host--pathogen interactions: a study of Acinetobacter baumannii responses to host airways.
Article in BMC genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- Quantitative Proteomic Analysis of Differential Proteins from Acinetobacter sp. HC8-3S Responses to Hydrocarbons.Current microbiology · 2026Article
- Goblet cell breakdown: transcriptomics reveals Acinetobacter baumannii early and robust inflammatory response in differentiated human bronchial epithelial cells.Journal of biomedical science · 2025Article
- Article
- iTRAQ-Based Quantitative Proteomic Analysis of Acinetobacter baumannii under Hypoxia and Normoxia Reveals the Role of OmpW as a Virulence Factor.Microbiology spectrum · 2022Article
- Correlative proteomics identify the key roles of stress tolerance strategies in Acinetobacter baumannii in response to polymyxin and human macrophages.PLoS pathogens · 2022Article
- Whole-cell vaccine candidates induce a protective response against virulentFrontiers in immunology · 2022Article
- Designing Multi-Antigen Vaccines AgainstFrontiers in immunology · 2021Article
- Microevolution in the major outer membrane protein OmpA ofMicrobial genomics · 2020Article
- Human pleural fluid triggers global changes in the transcriptional landscape of Acinetobacter baumannii as an adaptive response to stress.Scientific reports · 2019Article
- Unified feature association networks through integration of transcriptomic and proteomic data.PLoS computational biology · 2019Article
- Acinetobacter spp. porin Omp33-36: Classification and transcriptional response to carbapenems and host cells.PloS one · 2018Article
- Changed Expression of Cytoskeleton Proteins During Lung Injury in a Mouse Model ofFrontiers in microbiology · 2018Article
- Growth Rate and Biofilm Formation Ability of Clinical and Laboratory-Evolved Colistin-Resistant Strains ofFrontiers in microbiology · 2018Article
- Pneumonia infection in mice reveals the involvement of the feoA gene in the pathogenesis of Acinetobacter baumannii.Virulence · 2018Article
- Analysis of Differentially Expressed Proteins inBioMed research international · 2017Article
- Future Directions and Molecular Basis of Ventilator Associated Pneumonia.Canadian respiratory journal · 2017Review
- Proteomics As a Tool for Studying Bacterial Virulence and Antimicrobial Resistance.Frontiers in microbiology · 2016Review
- Proteomic analysis of symbiotic proteins of Glomus mosseae and Amorpha fruticosa.Scientific reports · 2015Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAcinetobacter baumannii is a major health problem. The most common infection caused by A. baumannii is hospital acquired pneumonia, and the associated mortality rate is approximately 50%. Neither in vivo nor ex vivo expression profiling has been performed at the proteomic or transcriptomic level for pneumonia caused by A. baumannii. In this study, we characterized the proteome of A. baumannii under conditions that simulate those found in the airways, to gain some insight into how A. baumannii adapts to the host and to improve knowledge about the pathogenesis and virulence of this bacterium. A clinical strain of A. baumannii was grown under different conditions: in the presence of bronchoalveolar lavage fluid from infected rats, of RAW 264.7 cells to simulate conditions in the respiratory tract and in control conditions. We used iTRAQ labelling and LC-MALDI-TOF/TOF to investigate how A. baumannii responds on exposure to macrophages/BALF.
results179 proteins showed differential expression. In both models, proteins involved in the following processes were over-expressed: (i) pathogenesis and virulence (OmpA, YjjK); (ii) cell wall/membrane/envelope biogenesis (MurC); (iii) energy production and conversion (acetyl-CoA hydrolase); and (iv) translation (50S ribosomal protein L9). Proteins involved in the following were under-expressed: (i) lipid metabolism (short-chain dehydrogenase); (ii) amino acid metabolism and transport (aspartate aminotransferase); (iii) unknown function (DNA-binding protein); and (iv) inorganic ion transport and metabolism (hydroperoxidase).
conclusionsWe observed alterations in cell wall synthesis and identified 2 upregulated virulence-associated proteins with >15 peptides/protein in both ex vivo models (OmpA and YjjK), suggesting that these proteins are fundamental for pathogenesis and virulence in the airways. This study is the first comprehensive overview of the ex vivo proteome of A. baumannii and is an important step towards identification of diagnostic biomarkers, novel drug targets and potential vaccine candidates in the fight against pneumonia caused by A. baumannii.
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