Evidence map›Paper›PMID 26025051›Full record

ArticlePlant physiology2015

The Arabidopsis GAGA-Binding Factor BASIC PENTACYSTEINE6 Recruits the POLYCOMB-REPRESSIVE COMPLEX1 Component LIKE HETEROCHROMATIN PROTEIN1 to GAGA DNA Motifs.

Andreas Hecker, Luise H Brand, Sébastien Peter, Nathalie Simoncello, Joachim Kilian, Klaus Harter, Valérie Gaudin, Dierk Wanke

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.

0numbers the graph read from it
0cells of the map it votes in
72citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

72 citing papers in PubMed.

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12 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Andreas HeckerCenter for Plant Molecular Biology, Plant Physiology, and Biophysical Chemistry, University of Tübingen, 72076 Tuebingen, Germany (A.H., L.H.B., S.P., J.K., K.H., D.W.);Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1318 AgroParisTech, Institut J.-P. Bourgin, Institut National de la Recherche Agronomique Centre de Versailles-Grignon, F-78026 Versailles, France (N.S., V.G.); andUniversität des Saarlandes, Molekulare Pflanzenbiologie, 66123 Saarbruecken, Germany (D.W.).
Luise H BrandCenter for Plant Molecular Biology, Plant Physiology, and Biophysical Chemistry, University of Tübingen, 72076 Tuebingen, Germany (A.H., L.H.B., S.P., J.K., K.H., D.W.);Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1318 AgroParisTech, Institut J.-P. Bourgin, Institut National de la Recherche Agronomique Centre de Versailles-Grignon, F-78026 Versailles, France (N.S., V.G.); andUniversität des Saarlandes, Molekulare Pflanzenbiologie, 66123 Saarbruecken, Germany (D.W.).
Sébastien PeterCenter for Plant Molecular Biology, Plant Physiology, and Biophysical Chemistry, University of Tübingen, 72076 Tuebingen, Germany (A.H., L.H.B., S.P., J.K., K.H., D.W.);Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1318 AgroParisTech, Institut J.-P. Bourgin, Institut National de la Recherche Agronomique Centre de Versailles-Grignon, F-78026 Versailles, France (N.S., V.G.); andUniversität des Saarlandes, Molekulare Pflanzenbiologie, 66123 Saarbruecken, Germany (D.W.).
Nathalie SimoncelloCenter for Plant Molecular Biology, Plant Physiology, and Biophysical Chemistry, University of Tübingen, 72076 Tuebingen, Germany (A.H., L.H.B., S.P., J.K., K.H., D.W.);Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1318 AgroParisTech, Institut J.-P. Bourgin, Institut National de la Recherche Agronomique Centre de Versailles-Grignon, F-78026 Versailles, France (N.S., V.G.); andUniversität des Saarlandes, Molekulare Pflanzenbiologie, 66123 Saarbruecken, Germany (D.W.).
Joachim KilianCenter for Plant Molecular Biology, Plant Physiology, and Biophysical Chemistry, University of Tübingen, 72076 Tuebingen, Germany (A.H., L.H.B., S.P., J.K., K.H., D.W.);Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1318 AgroParisTech, Institut J.-P. Bourgin, Institut National de la Recherche Agronomique Centre de Versailles-Grignon, F-78026 Versailles, France (N.S., V.G.); andUniversität des Saarlandes, Molekulare Pflanzenbiologie, 66123 Saarbruecken, Germany (D.W.).
Klaus HarterCenter for Plant Molecular Biology, Plant Physiology, and Biophysical Chemistry, University of Tübingen, 72076 Tuebingen, Germany (A.H., L.H.B., S.P., J.K., K.H., D.W.);Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1318 AgroParisTech, Institut J.-P. Bourgin, Institut National de la Recherche Agronomique Centre de Versailles-Grignon, F-78026 Versailles, France (N.S., V.G.); andUniversität des Saarlandes, Molekulare Pflanzenbiologie, 66123 Saarbruecken, Germany (D.W.).ORCID http://orcid.org/0000-0002-2150-6970
Valérie GaudinCenter for Plant Molecular Biology, Plant Physiology, and Biophysical Chemistry, University of Tübingen, 72076 Tuebingen, Germany (A.H., L.H.B., S.P., J.K., K.H., D.W.);Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1318 AgroParisTech, Institut J.-P. Bourgin, Institut National de la Recherche Agronomique Centre de Versailles-Grignon, F-78026 Versailles, France (N.S., V.G.); andUniversität des Saarlandes, Molekulare Pflanzenbiologie, 66123 Saarbruecken, Germany (D.W.).
Dierk WankeCenter for Plant Molecular Biology, Plant Physiology, and Biophysical Chemistry, University of Tübingen, 72076 Tuebingen, Germany (A.H., L.H.B., S.P., J.K., K.H., D.W.);Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1318 AgroParisTech, Institut J.-P. Bourgin, Institut National de la Recherche Agronomique Centre de Versailles-Grignon, F-78026 Versailles, France (N.S., V.G.); andUniversität des Saarlandes, Molekulare Pflanzenbiologie, 66123 Saarbruecken, Germany (D.W.) dierk.wanke@zmbp.uni-tuebingen.de.ORCID http://orcid.org/0000-0002-7083-1550

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Polycomb-repressive complexes (PRCs) play key roles in development by repressing a large number of genes involved in various functions. Much, however, remains to be discovered about PRC-silencing mechanisms as well as their targeting to specific genomic regions. Besides other mechanisms, GAGA-binding factors in animals can guide PRC members in a sequence-specific manner to Polycomb-responsive DNA elements. Here, we show that the Arabidopsis (Arabidopsis thaliana) GAGA-motif binding factor protein basic pentacysteine6 (BPC6) interacts with like heterochromatin protein1 (LHP1), a PRC1 component, and associates with vernalization2 (VRN2), a PRC2 component, in vivo. By using a modified DNA-protein interaction enzyme-linked immunosorbant assay, we could show that BPC6 was required and sufficient to recruit LHP1 to GAGA motif-containing DNA probes in vitro. We also found that LHP1 interacts with VRN2 and, therefore, can function as a possible scaffold between BPC6 and VRN2. The lhp1-4 bpc4 bpc6 triple mutant displayed a pleiotropic phenotype, extreme dwarfism and early flowering, which disclosed synergistic functions of LHP1 and group II plant BPC members. Transcriptome analyses supported this synergy and suggested a possible function in the concerted repression of homeotic genes, probably through histone H3 lysine-27 trimethylation. Hence, our findings suggest striking similarities between animal and plant GAGA-binding factors in the recruitment of PRC1 and PRC2 components to Polycomb-responsive DNA element-like GAGA motifs, which must have evolved through convergent evolution.

Indexed as

ArabidopsisArabidopsis ProteinsCarrier ProteinsChromosomal Proteins, Non-HistoneDNA-Binding ProteinsGene Expression Regulation, PlantGenetic LociGenetic PleiotropyHistonesLysineMethylationModels, BiologicalMutationNuclear ProteinsNucleotide MotifsPolycomb-Group ProteinsArabidopsis ProteinsAt5g17690 protein, ArabidopsisBPC6 protein, ArabidopsisCarrier ProteinsChromosomal Proteins, Non-HistoneDNA-Binding ProteinsHistonesLysineNuclear ProteinsPolycomb-Group ProteinsVRN2 protein, Arabidopsis

Identifiers

PMID26025051
PMCPMC4741334

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