ArticleBMC biotechnology2015
Application of M13 phage display for identifying immunogenic proteins from tick (Ixodes scapularis) saliva.
Article in BMC biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- RmTIL, a cysteine-rich protein as a putative microbiota modulator from Rhipicephalus microplus tick.Parasitology research · 2026Article
- Cattle Immunization with T7 Phage-Displayed Whole-Tick Antigens ReducesPathogens (Basel, Switzerland) · 2026Article
- Article
- Review
- Identification and Structure of Epitopes on Cashew Allergens Ana o 2 and Ana o 3 Using Phage Display.Molecules (Basel, Switzerland) · 2023Article
- Biomarker Discovery by ORFeome Phage Display.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Review
- TickSialoFam (TSFam): A Database That Helps to Classify Tick Salivary Proteins, a Review on Tick Salivary Protein Function and Evolution, With Considerations on the Tick Sialome Switching Phenomenon.Frontiers in cellular and infection microbiology · 2020Review
- The Central Role of Salivary Metalloproteases in Host Acquired Resistance to Tick Feeding.Frontiers in cellular and infection microbiology · 2020Article
- Discovery of Leptospira spp. seroreactive peptides using ORFeome phage display.PLoS neglected tropical diseases · 2019Article
- Mining gut microbiome oligopeptides by functional metaproteome display.Scientific reports · 2016Article
- Phage display as a promising approach for vaccine development.Journal of biomedical science · 2016Review
- Continuous microfluidic assortment of interactive ligands (CMAIL).Scientific reports · 2016Article
- Ixodes scapularis Tick Saliva Proteins Sequentially Secreted Every 24 h during Blood Feeding.PLoS neglected tropical diseases · 2016Article
- Fructose 1,6-Bisphosphate Aldolase, a Novel Immunogenic Surface Protein on Listeria Species.PloS one · 2016Article
- Identification of Novel Immunogenic Proteins of Neisseria gonorrhoeae by Phage Display.PloS one · 2016Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundTicks act as vectors for a large number of different pathogens, perhaps most notably Borrelia burgdorferi, the causative agent of Lyme disease. The most prominent tick vector in the United States is the blacklegged tick, Ixodes scapularis. Tick bites are of special public health concern since there are no vaccines available against most tick-transmitted pathogens. Based on the observation that certain non-natural host animals such as guinea pigs or humans can develop adaptive immune responses to tick bites, anti-tick vaccination is a potential approach to tackle health risks associated with tick bites.
resultsThe aim of this study was to use an oligopeptide phage display strategy to identify immunogenic salivary gland proteins from I. scapularis that are recognized by human immune sera. Oligopeptide libraries were generated from salivary gland mRNA of 18 h fed nymphal I. scapularis. Eight immunogenic oligopeptides were selected using human immune sera. Three selected immunogenic oligopeptides were cloned and produced as recombinant proteins. The immunogenic character of an identified metalloprotease (MP1) was validated with human sera. This enzyme has been described previously and was hypothesized as immunogenic which was confirmed in this study. Interestingly, it also has close homologs in other Ixodes species.
conclusionAn immunogenic protein of I. scapularis was identified by oligopeptide phage display. MP1 is a potential candidate for vaccine development.
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