ArticlePloS one2015
Superovulation using the combined administration of inhibin antiserum and equine chorionic gonadotropin increases the number of ovulated oocytes in C57BL/6 female mice.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed, 125 citations in OpenAlex.
- Repeated surgical oocyte collection from severe immunodeficient NOG mice after repeated superovulation with inhibin antiserum and eCG.Experimental animals · 2026Article
- Preparation of Pseudopregnant Recipient Females Using Female Mice (Mus musculus) Showing Nonestrous Signs: Investigation of a Unique Approach Based on the Whitten Effect.Journal of the American Association for Laboratory Animal Science : JAALAS · 2026Article
- Cryopreservation of Rodent Ovarian Tissues and Oocytes.Methods in molecular biology (Clifton, N.J.) · 2026Article
- An improved vitrification protocol for the fast and safe storage of mouse oocytes†.Biology of reproduction · 2025Article
- Developmental Potential of In Vitro Fertilization-Derived Mouse Zygotes Following Vitrification: Effects of Superovulation Method.Journal of the American Association for Laboratory Animal Science : JAALAS · 2025Article
- Article
- 17β-estradiol and estrogen receptor alpha protect mouse ovarian follicle development by repressing atresia.iScience · 2025Article
- Systematic identification of Y-chromosome gene functions in mouse spermatogenesis.Science (New York, N.Y.) · 2025Article
- Screening for novel factors involved in mouse early embryonic development using inhibitor libraries.Frontiers in cell and developmental biology · 2025Article
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- Effect of ovarian stimulation on developmental speed of preimplantation embryo in a mouse model.The Journal of reproduction and development · 2024Article
- Thirteen Ovary-Enriched Genes Are Individually Not Essential for Female Fertility in Mice.Cells · 2024Article
- CYRI-B-mediated macropinocytosis drives metastasis via lysophosphatidic acid receptor uptake.eLife · 2024Article
- Superovulation with an anti-inhibin monoclonal antibody improves the reproductive performance of rat strains by increasing the pregnancy rate and the litter size.Scientific reports · 2024Article
- Aberrant CD8Frontiers in immunology · 2024Article
- Generation of Meiotic Mouse Models Using CRISPR/Cas9 Technology.Methods in molecular biology (Clifton, N.J.) · 2024Article
- A combined treatment with progesterone, anti-inhibin serum, and equine chorionic gonadotropin improves number of ovulated oocytes in young C57BL/6J mice.The Journal of reproduction and development · 2023Article
- Article
- Gene Editing in Mouse Zygotes Using the CRISPR/Cas9 System.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Practical Application of the 3Rs in Rodent Transgenesis.Methods in molecular biology (Clifton, N.J.) · 2023Article
5 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Superovulation is a reproductive technique generally used to produce genetically engineered mice. Superovulation in mice involves the administration of equine chorionic gonadotropin (eCG) to promote follicle growth and then that of human chorionic gonadotropin (hCG) to induce ovulation. Previously, some published studies reported that inhibin antiserum (IAS) increased the number of ovulated oocytes in ddY and wild-derived strains of mice. However, the effect of IAS on the C57BL/6 strain, which is the most widely used inbred strain for the production of genetically engineered mice, has not been investigated. In addition, the combined effect of IAS and eCG (IASe) on the number of ovulated oocytes in superovulation treatment has not been examined. In this study, we examined the effect of IAS and eCG on the number of ovulated oocytes in immature female mice of the C57BL/6 strain in superovulation treatment. Furthermore, we evaluated the quality of obtained oocytes produced by superovulation using IASe by in vitro fertilization (IVF) with sperm from C57BL/6 or genetically engineered mice. The developmental ability of fresh or cryopreserved embryos was examined by embryo transfer. The administration of IAS or eCG had a similar effect on the number of ovulated oocytes in C57BL/6 female mice. The number of ovulated oocytes increased to about 3-fold by the administration of IASe than by the administration of IAS or eCG alone. Oocytes derived from superovulation using IASe normally developed into 2-cell embryos by IVF using sperm from C57BL/6 mice. Fresh or cryopreserved 2-cell embryos produced by IVF between oocytes of C57BL/6 mice and sperm from genetically engineered mice normally developed into live pups following embryo transfer. In summary, a novel technique of superovulation using IASe is extremely useful for producing a great number of oocytes and offspring from genetically engineered mice.
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