ArticleThe Journal of biological chemistry2015
Generation of Dopamine Neurons from Rodent Fibroblasts through the Expandable Neural Precursor Cell Stage.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- Y-shaped trivalent aptamer for targeted visualization and tracking of reprogrammed astrocytes.Materials today. Bio · 2025Article
- In Vitro Modeling as a Tool for Testing Therapeutics for Spinal Muscular Atrophy and IGHMBP2-Related Disorders.Biology · 2023Article
- Roles of Transcription Factors in the Development and Reprogramming of the Dopaminergic Neurons.International journal of molecular sciences · 2022Review
- Direct Neuronal Reprogramming: Bridging the Gap Between Basic Science and Clinical Application.Frontiers in cell and developmental biology · 2021Review
- Generation of Induced Dopaminergic Neurons from Human Fetal Fibroblasts.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Direct conversion of human fibroblasts into dopaminergic neuron-like cells using small molecules and protein factors.Military Medical Research · 2020Article
- Acquisition of the Midbrain Dopaminergic Neuronal Identity.International journal of molecular sciences · 2020Review
- Review
- Hybrid Nanofiber Scaffold-Based Direct Conversion of Neural Precursor Cells/Dopamine Neurons.International journal of stem cells · 2019Article
- Lethal Factor Domain-Mediated Delivery of Nurr1 Transcription Factor Enhances Tyrosine Hydroxylase Activity and Protects from Neurotoxin-Induced Degeneration of Dopaminergic Cells.Molecular neurobiology · 2019Article
- Generation of dopamine neuronal-like cells from induced neural precursors derived from adult human cells by non-viral expression of lineage factors.Journal of stem cells & regenerative medicine · 2018Article
- Efficient Generation of Dopamine Neurons by Synthetic Transcription Factor mRNAs.Molecular therapy : the journal of the American Society of Gene Therapy · 2017Article
- Comparison of Reprogramming Methods for Generation of Induced-Oligodendrocyte Precursor Cells.Biomolecules & therapeutics · 2017Article
- Understanding Parkinson's Disease through the Use of Cell Reprogramming.Stem cell reviews and reports · 2017Review
- Induced dopaminergic neurons: A new promise for Parkinson's disease.Redox biology · 2017Article
- Efficient induction of neural precursor cells from fibroblasts using stromal cell-derived inducing activity.Tissue engineering and regenerative medicine · 2016Article
- Directed differentiation of postnatal hippocampal neural stem cells generates nuclear receptor related‑1 protein‑ and tyrosine hydroxylase‑expressing cells.Molecular medicine reports · 2016Article
- FGF8 is Essential for Functionality of Induced Neural Precursor Cell-derived Dopaminergic Neurons.International journal of stem cells · 2015Article
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent groundbreaking work has demonstrated that combined expression of the transcription factors Brn2, Ascl1, and Myt1L (BAM; also known as Wernig factors) convert mouse fibroblasts into postmitotic neuronal cells. However, questions remain regarding whether trans-conversion is achieved directly or involves an intermediary precursor stage. Trans-conversion toward expandable neural precursor cells (NPCs) is more useful than direct one-step neuron formation with respect to yielding a sufficient number of cells and the feasibility of manipulating NPC differentiation toward certain neuron subtypes. Here, we show that co-expression of Wernig factors and Bcl-xL induces fibroblast conversion into NPCs (induced NPCs (iNPCs)) that are highly expandable for >100 passages. Gene expression analyses showed that the iNPCs exhibited high expression of common NPC genes but not genes specific to defined embryonic brain regions. This finding indicated that a regional identity of iNPCs was not established. Upon induction, iNPCs predominantly differentiated into astrocytes. However, the differentiation potential was not fixed and could be efficiently manipulated into general or specific subtypes of neurons by expression of additional genes. Specifically, overexpression of Nurr1 and Foxa2, transcription factors specific for midbrain dopamine neuron development, drove iNPCs to yield mature midbrain dopamine neurons equipped with presynaptic DA neuronal functions. We further assessed the therapeutic potential of iNPCs in Parkinson disease model rats.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.