Evidence map›Paper›PMID 26022764›Full record

ArticleRegenerative medicine2015

Increased yield of endothelial cells from peripheral blood for cell therapies and tissue engineering.

Ryan M Jamiolkowski, Sa Do Kang, AnnMarie K Rodriguez, Justin M Haseltine, Lauren J Galinat, Alexandra E Jantzen, Tim A Carlon, Marcus D Darrabie, Antonio J Arciniegas, Jose G Mantilla and 11 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Regenerative medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.0field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors at 4 institutions in 3 countries.

Ryan M Jamiolkowski1Department of Surgery, Duke University Medical Center, NC, USA.
Sa Do Kang1Department of Surgery, Duke University Medical Center, NC, USA.
AnnMarie K Rodriguez1Department of Surgery, Duke University Medical Center, NC, USA.
Justin M Haseltine1Department of Surgery, Duke University Medical Center, NC, USA.
Lauren J Galinat1Department of Surgery, Duke University Medical Center, NC, USA.
Alexandra E Jantzen2Department of Biomedical Engineering, Duke University, NC, USA.
Tim A Carlon1Department of Surgery, Duke University Medical Center, NC, USA.
Marcus D Darrabie1Department of Surgery, Duke University Medical Center, NC, USA.
Antonio J Arciniegas1Department of Surgery, Duke University Medical Center, NC, USA.
Jose G Mantilla1Department of Surgery, Duke University Medical Center, NC, USA.
N Rebecca Haley3Duke Translational Medicine Institute, NC, USA.
Maria Noviani1Department of Surgery, Duke University Medical Center, NC, USA.
Jason D Allen5Department of Community and Family Medicine, Duke University Medical Center, NC, USA.
Thomas V Stabler6Duke Molecular Physiology Institute, Duke University Medical Center, NC, USA.
James W Frederiksen1Department of Surgery, Duke University Medical Center, NC, USA.
Oscar Alzate8University of North Carolina, School of Medicine, NC, USA.
Lukas G Keil8University of North Carolina, School of Medicine, NC, USA.
Siyao Liu8University of North Carolina, School of Medicine, NC, USA.
Fu-Hsiung Lin1Department of Surgery, Duke University Medical Center, NC, USA.
George A Truskey2Department of Biomedical Engineering, Duke University, NC, USA.
Hardean E Achneck1Department of Surgery, Duke University Medical Center, NC, USA.
Duke Medical Center · USDuke University · USDuke University Hospital · USVictoria University · AU

Funding

Endothelial Progenitor Cell Seeded Self-Expanding Vascular StentR21HL109897 · NHLBI · DUKE UNIVERSITY · PI ACHNECK, HARDEAN ERIC · 2011 to 2012
$432k
NHLBI NIH HHS R21 HL109897NHLBI NIH HHS R21 HL109897-01
6 · The paper itself

Abstract

aimPeripheral blood-derived endothelial cells (pBD-ECs) are an attractive tool for cell therapies and tissue engineering, but have been limited by their low isolation yield. We increase pBD-EC yield via administration of the chemokine receptor type 4 antagonist AMD3100, as well as via a diluted whole blood incubation (DWBI). MATERIALS &

methodsPorcine pBD-ECs were isolated using AMD3100 and DWBI and tested for EC markers, acetylated LDL uptake, growth kinetics, metabolic activity, flow-mediated nitric oxide production and seeded onto titanium tubes implanted into vessels of pigs.

resultsDWBI increased the yield of porcine pBD-ECs 6.6-fold, and AMD3100 increased the yield 4.5-fold. AMD3100-mobilized ECs were phenotypically indistinguishable from nonmobilized ECs. In porcine implants, the cells expressed endothelial nitric oxide synthase, reduced thrombin-antithrombin complex systemically and prevented thrombosis.

conclusionAdministration of AMD3100 and the DWBI method both increase pBD-EC yield.

Indexed as

AnimalsBenzylaminesCell SeparationCell TransplantationCyclamsEndothelial CellsFlow CytometryHeterocyclic CompoundsModels, AnimalRheologyStress, MechanicalSus scrofaTissue EngineeringTransplantation, AutologousVena Cava, InferiorBenzylaminesCyclamsHeterocyclic CompoundsplerixaforAMD3100biocompatible materialscell Isolationcell therapyendothelial cellsendothelial progenitor cellsswinethrombosistissue engineering

Identifiers

PMID26022764
PMCPMC4489558
OpenAlexW1918728123

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.