Evidence map›Paper›PMID 26022362›Full record

ArticleNeuroscience2015

The inhibition of high-voltage-activated calcium current by activation of MrgC11 involves phospholipase C-dependent mechanisms.

Z Li, S-Q He, P-Y Tseng, Q Xu, V Tiwari, F Yang, B Shu, T Zhang, Z Tang, S N Raja and 3 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuroscience, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
0.8field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 14 citations in OpenAlex.

  1. Inhibitors of Phosphatidylinositol-specific Phospholipase C wit hMedicinal chemistry (Shariqah (United Arab Emirates)) · 2026
    Article
  2. Article
  3. Review
  4. Frontiers in pain research (Lausanne, Switzerland) · 2021
    Article
  5. Article
  6. Article
  7. Nociceptor Signalling through ion Channel Regulation via GPCRs.International journal of molecular sciences · 2019
    Review
  8. Article
  9. Targeting human Mas-related G protein-coupled receptor X1 to inhibit persistent pain.Proceedings of the National Academy of Sciences of the United States of America · 2017
    Article
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 6 institutions in 2 countries.

Z LiThe Solomon H. Snyder Department of Neuroscience, Center for Sensory Biology, Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.
S-Q HeDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.
P-Y TsengThe Solomon H. Snyder Department of Neuroscience, Center for Sensory Biology, Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.
Q XuThe Solomon H. Snyder Department of Neuroscience, Center for Sensory Biology, Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.
V TiwariDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.
F YangDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.
B ShuDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA; Department of Anesthesiology, Tongji Hospital, Tongji Medical College, Wuhan 430030, China.
T ZhangDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA; Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China.
Z TangNanjing University of Chinese Medicine, 138 Xianlin Road, Nanjing, Jiangsu 210023, China.
S N RajaDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.
Y WangDepartment of Anesthesiology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China.
X DongThe Solomon H. Snyder Department of Neuroscience, Center for Sensory Biology, Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA; Howard Hughes Medical Institute, Johns Hopkins University, School of Medicine, Baltimore, MD 21287, USA. Electronic address: xdong2@jhmi.edu.
Y GuanDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA. Electronic address: yguan1@jhmi.edu.
Johns Hopkins Medicine · USJohns Hopkins University · USBeijing Tian Tan Hospital · CNCapital Medical University · CNNanjing University of Chinese Medicine · CNTongji Hospital · CN

Funding

INJURY-INDUCES PAIN-- CHEMICAL MODULATION OF NOCICEPTORSR01NS026363 · NINDS · JOHNS HOPKINS UNIVERSITY · PI RAJA, SRINIVASA N. · 1989 to 2021
$6.8M
Functional analysis of the Mrg receptor family in pain sensationR01NS054791 · NINDS · JOHNS HOPKINS UNIVERSITY · PI DONG, XINZHONG · 2007 to 2020
$5.0M
Mrgs as New Target for the Treatment of Neuropathic PainR01NS070814 · NINDS · JOHNS HOPKINS UNIVERSITY · PI GUAN, YUN · 2011 to 2020
$3.2M
Functional Analysis of Mrgpr Family in itch sensationR37NS054791 · NINDS · JOHNS HOPKINS UNIVERSITY · PI Xinzhong Dong · 2022 to 2026
$2.9M
Howard Hughes Medical InstituteNINDS NIH HHS NS26363NINDS NIH HHS NS54791NINDS NIH HHS NS70814NINDS NIH HHS R01 NS026363NINDS NIH HHS R01 NS054791NINDS NIH HHS R01 NS070814NINDS NIH HHS R37 NS054791
6 · The paper itself

Abstract

High-voltage-activated (HVA) calcium channels play an important role in synaptic transmission. Activation of Mas-related G-protein-coupled receptor subtype C (MrgC; mouse MrgC11, rat homolog rMrgC) inhibits HVA calcium current (ICa) in small-diameter dorsal root ganglion (DRG) neurons, but the intracellular signaling cascade underlying MrgC agonist-induced inhibition of HVA ICa in native DRG neurons remains unclear. To address this question, we conducted patch-clamp recordings in MrgA3-eGFP-wild-type mice, in which most MrgA3-eGFP(+) DRG neurons co-express MrgC11 and can be identified for recording. We found that the inhibition of HVA ICa by JHU58 (0.001-100nM, a dipeptide, MrgC-selective agonist) was significantly reduced by pretreatment with a phospholipase C (PLC) inhibitor (U73122, 1μM), but not by its inactive analog (U73343) or vehicle. Further, in rats that had undergone spinal nerve injury, pretreatment with intrathecal U73122 nearly abolished the inhibition of mechanical hypersensitivity by intrathecal JHU58. The inhibition of HVA ICa in MrgA3-eGFP(+) neurons by JHU58 (100nM) was partially reduced by pretreatment with a Gβγ blocker (gallein, 100μM). However, applying a depolarizing prepulse and blocking the Gαi and Gαs pathways with pertussis toxin (PTX) (0.5μg/mL) and cholera toxin (CTX) (0.5μg/mL), respectively, had no effect. These findings suggest that activation of MrgC11 may inhibit HVA ICa in mouse DRG neurons through a voltage-independent mechanism that involves activation of the PLC, but not Gαi or Gαs, pathway.

Indexed as

AnimalsCalcium ChannelsCells, CulturedDisease Models, AnimalGanglia, SpinalGreen Fluorescent ProteinsHyperalgesiaMaleMice, KnockoutMice, TransgenicNeuronsPatch-Clamp TechniquesRats, Sprague-DawleyReceptors, G-Protein-CoupledSpinal NervesType C PhospholipasesCalcium ChannelsGreen Fluorescent ProteinsMrgprc protein, ratMrgprx1 protein, mouseReceptors, G-Protein-CoupledType C Phospholipasescalcium channeldorsal root ganglionMrgCpainPLC

Identifiers

PMID26022362
PMCPMC4485588
OpenAlexW359130503

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.