ArticleScientific reports2015
Non-linear optical flow cytometry using a scanned, Bessel beam light-sheet.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed.
- Multicolor iLIFE (m-iLIFE) volume cytometry for high-throughput imaging of multiple organelles.Scientific reports · 2024Article
- Light sheet based volume flow cytometry (VFC) for rapid volume reconstruction and parameter estimation on the go.Scientific reports · 2022Article
- An intrinsic, label-free signal for identifying stem cell-derived cardiomyocyte subtype.Stem cells (Dayton, Ohio) · 2020Article
- Optical second harmonic generation microscopy: application to the sensitive detection of cell membrane damage.Biophysical reviews · 2019Review
- Article
- Nanolithography using Bessel Beams of Extreme Ultraviolet Wavelength.Scientific reports · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Modern flow cytometry instruments have become vital tools for high-throughput analysis of single cells. However, as issues with the cellular labeling techniques often used in flow cytometry have become more of a concern, the development of label-free modalities for cellular analysis is increasingly desired. Non-linear optical phenomena (NLO) are of growing interest for label-free analysis because of the ability to measure the intrinsic optical response of biomolecules found in cells. We demonstrate that a light-sheet consisting of a scanned Bessel beam is an optimal excitation geometry for efficiently generating NLO signals in a microfluidic environment. The balance of photon density and cross-sectional area provided by the light-sheet allowed significantly larger two-photon fluorescence intensities to be measured in a model polystyrene microparticle system compared to measurements made using other excitation focal geometries, including a relaxed Gaussian excitation beam often used in conventional flow cytometers.
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Registered trials
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