Evidence map›Paper›PMID 26021570›Full record

ArticlePlasmid2015

A series of medium and high copy number arabinose-inducible Escherichia coli expression vectors compatible with pBR322 and pACYC184.

Vandana Chakravartty, John E Cronan

Open access · greenAbstract read
In one paragraph

Article in Plasmid, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.5field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 23 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Physiological Roles of anbioRxiv : the preprint server for biology · 2024
    Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Protein moonlighting elucidates the essential human pathway catalyzing lipoic acid assembly on its cognate enzymes.Proceedings of the National Academy of Sciences of the United States of America · 2018
    Article
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 1 country.

Vandana ChakravarttyDepartment of Microbiology, University of Illinois, B103 Chemical and Life Sciences Laboratory, 601S. Goodwin Ave., Urbana, IL 61801, USA.
John E CronanDepartment of Microbiology, University of Illinois, B103 Chemical and Life Sciences Laboratory, 601S. Goodwin Ave., Urbana, IL 61801, USA; Department of Biochemistry, University of Illinois, 419 Roger Adams Laboratory, 600 South Mathews Ave., Urbana, IL 61801, USA. Electronic address: j-cronan@life.uiuc.edu.
Goodwin College · USUniversity of Illinois Urbana-Champaign · US

Funding

GENETIC APPROACHES TO REGULATION OF LIPID METABOLISMR01AI015650 · NIAID · UNIVERSITY OF ILLINOIS URBANA-CHAMPAIGN · PI CRONAN, JOHN E. · 1985 to 2022
$12.0M
GENETIC APPROACHES TO REGULATION OF LIPID METABOLISMR37AI015650 · NIAID · UNIVERSITY OF ILLINOIS URBANA-CHAMPAIGN · PI CRONAN, JOHN E. · 1994 to 2003
$1.8M
NIAID NIH HHS AI15650NIAID NIH HHS R01 AI015650NIAID NIH HHS R37 AI015650
6 · The paper itself

Abstract

The original pBAD24 plasmid and the derived lower copy number (the pBAD322 series) expression vectors have been widely used in Escherichia coli, Salmonella enterica, and related bacteria. However, a flexible pBAD expression system has been available only in pMB1 (ColE1) vectors. We report a series of pBAD vectors that replicate using the origin of plasmid RSF1030 that are compatible with pMB1 (ColE1) and p15A (pACYC) vectors. Both high (≥pBAD24) and medium (~pBAD322) copy number plasmids encoding resistance to ampicillin, chloramphenicol, kanamycin, tetracycline, spectinomycin/streptomycin, gentamycin, or trimethoprim are available.

Indexed as

Gene DosageArabinoseCloning, MolecularEscherichia coliGene Expression Regulation, BacterialGenetic VectorsGenomic InstabilityPlasmidsArabinoseCopy numberExpression levelspBAD24pBR322Plasmid CompatibilityRSF1030

Identifiers

PMID26021570
PMCPMC4600428
OpenAlexW876659836

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.