ArticleJournal of cell science2015
Genome-wide RNAi screen for nuclear actin reveals a network of cofilin regulators.
Article in Journal of cell science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 40 citations in OpenAlex.
- Multilayered regulation of cytoskeletal protein abundance: autoregulatory mechanisms of actin and tubulin.Experimental & molecular medicine · 2026Review
- The O-GlcNAcylation of β-actin Ser199 controls nuclear speckle localization and is dysregulated in diabetes.Histochemistry and cell biology · 2025Article
- Review
- Reducing Cofilin dosage makes embryos resilient to heat stress.bioRxiv : the preprint server for biology · 2025Article
- Nuclear actin is a critical regulator ofbioRxiv : the preprint server for biology · 2024Article
- Soma-germline communication drives sex maintenance in theNational science review · 2024Article
- Regulation of Precise DNA Repair by Nuclear Actin Polymerization: A Chance for Improving Gene Therapy?Cells · 2024Review
- Neuroligin 2 governs synaptic morphology and function through RACK1-cofilin signaling in Drosophila.Communications biology · 2023Article
- Misregulation of Nucleoporins 98 and 96 leads to defects in protein synthesis that promote hallmarks of tumorigenesis.Disease models & mechanisms · 2022Article
- Review
- Emerging Properties and Functions of Actin and Actin Filaments Inside the Nucleus.Cold Spring Harbor perspectives in biology · 2021Review
- Article
- The Actin-Family Protein Arp4 Is a Novel Suppressor for the Formation and Functions of Nuclear F-Actin.Cells · 2020Article
- Nuclear architecture as an intrinsic regulator of Drosophila female germline stem cell maintenance.Current opinion in insect science · 2020Review
- Nuclear Actin: From Discovery to Function.Anatomical record (Hoboken, N.J. : 2007) · 2018Review
- Nuclear actin: ancient clue to evolution in eukaryotes?Histochemistry and cell biology · 2018Review
- Initiation of DNA replication requires actin dynamics and formin activity.The EMBO journal · 2017Article
- Nuclear Actin in Development and Transcriptional Reprogramming.Frontiers in genetics · 2017Review
- Fascin regulates nuclear actin during Drosophila oogenesis.Molecular biology of the cell · 2016Article
- Persistent nuclear actin filaments inhibit transcription by RNA polymerase II.Journal of cell science · 2016Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
Nuclear actin plays an important role in many processes that regulate gene expression. Cytoplasmic actin dynamics are tightly controlled by numerous actin-binding proteins, but regulation of nuclear actin has remained unclear. Here, we performed a genome-wide RNA interference (RNAi) screen in Drosophila cells to identify proteins that influence either nuclear polymerization or import of actin. We validate 19 factors as specific hits, and show that Chinmo (known as Bach2 in mammals), SNF4Aγ (Prkag1 in mammals) and Rab18 play a role in nuclear localization of actin in both fly and mammalian cells. We identify several new regulators of cofilin activity, and characterize modulators of both cofilin kinases and phosphatase. For example, Chinmo/Bach2, which regulates nuclear actin levels also in vivo, maintains active cofilin by repressing the expression of the kinase Cdi (Tesk in mammals). Finally, we show that Nup98 and lamin are candidates for regulating nuclear actin polymerization. Our screen therefore reveals new aspects of actin regulation and links nuclear actin to many cellular processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.