ArticlePLoS genetics2015
Dissecting the function and assembly of acentriolar microtubule organizing centers in Drosophila cells in vivo.
Article in PLoS genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- Ninein domains required for its localization, association with partners dynein and ensconsin, and microtubule organization.Molecular biology of the cell · 2024Article
- SAPs as a new model to probe the pathway of centriole and centrosome assembly.Biochemical Society transactions · 2021Review
- Centriole-independent mitotic spindle assembly relies on the PCNT-CDK5RAP2 pericentriolar matrix.The Journal of cell biology · 2020Article
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- Article
- Targeting centrosome amplification, an Achilles' heel of cancer.Biochemical Society transactions · 2019Review
- Actomyosin-Driven Tension at Compartmental Boundaries Orients Cell Division Independently of Cell Geometry In Vivo.Developmental cell · 2018Article
- PLK4 is a microtubule-associated protein that self-assembles promotingJournal of cell science · 2018Article
- The dual role of the centrosome in organizing the microtubule network in interphase.EMBO reports · 2018Article
- The chromosomal basis of meiotic acentrosomal spindle assembly and function in oocytes.Chromosoma · 2017Review
- Mitotic spindle assembly in animal cells: a fine balancing act.Nature reviews. Molecular cell biology · 2017Review
- Article
- Drosophila Ana1 is required for centrosome assembly and centriole elongation.Journal of cell science · 2016Article
- Cdk1 Phosphorylates Drosophila Sas-4 to Recruit Polo to Daughter Centrioles and Convert Them to Centrosomes.Developmental cell · 2016Article
- Centrosomes are multifunctional regulators of genome stability.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2016Review
- Microcephaly protein Asp focuses the minus ends of spindle microtubules at the pole and within the spindle.The Journal of cell biology · 2015Article
- Centrosome function and assembly in animal cells.Nature reviews. Molecular cell biology · 2015Review
- The Drosophila Pericentrin-like-protein (PLP) cooperates with Cnn to maintain the integrity of the outer PCM.Biology open · 2015Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Acentriolar microtubule organizing centers (aMTOCs) are formed during meiosis and mitosis in several cell types, but their function and assembly mechanism is unclear. Importantly, aMTOCs can be overactive in cancer cells, enhancing multipolar spindle formation, merotelic kinetochore attachment and aneuploidy. Here we show that aMTOCs can form in acentriolar Drosophila somatic cells in vivo via an assembly pathway that depends on Asl, Cnn and, to a lesser extent, Spd-2--the same proteins that appear to drive mitotic centrosome assembly in flies. This finding enabled us to ablate aMTOC formation in acentriolar cells, and so perform a detailed genetic analysis of the contribution of aMTOCs to acentriolar mitotic spindle formation. Here we show that although aMTOCs can nucleate microtubules, they do not detectably increase the efficiency of acentriolar spindle assembly in somatic fly cells. We find that they are required, however, for robust microtubule array assembly in cells without centrioles that also lack microtubule nucleation from around the chromatin. Importantly, aMTOCs are also essential for dynein-dependent acentriolar spindle pole focusing and for robust cell proliferation in the absence of centrioles and HSET/Ncd (a kinesin essential for acentriolar spindle pole focusing in many systems). We propose an updated model for acentriolar spindle pole coalescence by the molecular motors Ncd/HSET and dynein in conjunction with aMTOCs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.