ArticlePloS one2015
Chitosan enriched three-dimensional matrix reduces inflammatory and catabolic mediators production by human chondrocytes.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 38 citations in OpenAlex.
- Efficacy and safety of mesenchymal stem cell injections for patients with osteoarthritis: a meta-analysis and review of RCTs.Archives of orthopaedic and trauma surgery · 2021Pooled it
- Smart functionalized cartilage repair microspheres: Concepts and applications.Materials today. Bio · 2026Review
- Injectable rhBMP-2-loaded calcium phosphate cement and chitosan composite hydrogel for the repair of osteoporotic bone defects.BMC musculoskeletal disorders · 2026Article
- Comparison of Three Different Techniques for the Treatment of Cartilage Lesions-Matrix-Induced Autologous Chondrocyte Implantation (MACI) Versus Autologous Matrix-Induced Chondrogenesis (AMIC) and Arthroscopic Minced Cartilage-A 2-Year Follow-Up on Patient-Reported Pain and Functional Outcomes.Journal of clinical medicine · 2025Article
- Long-Term Efficacy of Carboxymethyl-Chitosan in Advanced Knee Osteoarthritis: A Twelve-Month Follow-Up Study on Non-Responders to Hyaluronic Acid.Biomedicines · 2025Article
- Hydrogel based scaffolds in cardiovascular disease: applications in myocardial regeneration, biological pacing, and heart failure therapy.Frontiers in cell and developmental biology · 2025Review
- Intra-articular injections with Carboxymethyl-Chitosan in patients affected by knee osteoarthritis non-responders to hyaluronic acid: a pilot study.European journal of translational myology · 2024Article
- Chitosan-based scaffold augmentation to microfractures: Stable results at mid-term follow-up in patients with patellar cartilage lesions.Journal of experimental orthopaedics · 2024Article
- Diacerein-loaded surface modified iron oxide microparticles (SMIOMPs): an emerging magnetic system for management of osteoarthritis via intra-articular injection.Frontiers in bioengineering and biotechnology · 2024Article
- Injectable Scaffold with Microfracture using the Autologous Matrix-Induced Chondrogenesis (AMIC) Technique: A Prospective Cohort Study.Malaysian orthopaedic journal · 2022Article
- Thermosensitive Injectable Hydrogels for Intra-Articular Delivery of Etanercept for the Treatment of Osteoarthritis.Gels (Basel, Switzerland) · 2022Article
- Anisotropic Chitosan Scaffolds Generated by Electrostatic Flocking Combined with Alginate Hydrogel Support Chondrogenic Differentiation.International journal of molecular sciences · 2021Article
- Cytotoxic and anti-inflammatory effects of chitosan and hemostatic gelatin in oral cell culture.Acta odontologica latinoamericana : AOL · 2021Article
- PHB/CHIT Scaffold as a Promising Biopolymer in the Treatment of Osteochondral Defects-An Experimental Animal Study.Polymers · 2021Article
- Versatile Use of Chitosan and Hyaluronan in Medicine.Molecules (Basel, Switzerland) · 2021Review
- Intra-articular Injection of Chitosan-Based Supramolecular Hydrogel for Osteoarthritis Treatment.Tissue engineering and regenerative medicine · 2021Article
- Chitosan and Lecithin Ameliorate Osteoarthritis Symptoms Induced by Monoiodoacetate in a Rat Model.Molecules (Basel, Switzerland) · 2020Article
- Preliminary evaluation of an osteochondral autograft, a prosthetic implant, and a biphasic absorbable implant for osteochondral reconstruction in a sheep model.Veterinary surgery : VS · 2020Article
- A Self-Setting Hydrogel of Silylated Chitosan and Cellulose for the Repair of Osteochondral Defects: FromFrontiers in bioengineering and biotechnology · 2020Article
- Chitosans for Tissue Repair and Organ Three-Dimensional (3D) Bioprinting.Micromachines · 2019Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This in vitro study investigated the metabolism of human osteoarthritic (OA) chondrocytes encapsulated in a spherical matrix enriched of chitosan. Human OA chondrocytes were encapsulated and cultured for 28 days either in chitosan-alginate beads or in alginate beads. The beads were formed by slowly passing dropwise either the chitosan 0.6%-alginate 1.2% or the alginate 1.2% solution through a syringe into a 102 mM CaCl2 solution. Beads were analyzed histologically after 28 days. Interleukin (IL)-6 and -8, prostaglandin (PG) E2, matrix metalloproteinases (MMPs), hyaluronan and aggrecan were quantified directly in the culture supernatant by specific ELISA and nitric oxide (NO) by using a colorimetric method based on the Griess reaction. Hematoxylin and eosin staining showed that chitosan was homogeneously distributed through the matrix and was in direct contact with chondrocytes. The production of IL-6, IL-8 and MMP-3 by chondrocytes significantly decreased in chitosan-alginate beads compared to alginate beads. PGE2 and NO decreased also significantly but only during the first three days of culture. Hyaluronan and aggrecan production tended to increase in chitosan-alginate beads after 28 days of culture. Chitosan-alginate beads reduced the production of inflammatory and catabolic mediators by OA chondrocytes and tended to stimulate the synthesis of cartilage matrix components. These particular effects indicate that chitosan-alginate beads are an interesting scaffold for chondrocytes encapsulation before transplantation to repair cartilage defects.
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