ArticlePloS one2015
Oviduct-specific expression of human neutrophil defensin 4 in lentivirally generated transgenic chickens.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 48 citations in OpenAlex.
- Feathered bioreactor: transforming a chicken egg into a recombinant protein biomanufacturing foundry.Poultry science · 2026Review
- Mechanisms and Implications of Gut Microbiota-Derived Metabolites in the Regulation of Poultry Defensins.Animals : an open access journal from MDPI · 2026Review
- Association between miRNAs and DEFA4 in coronary artery disease.Molecular biology reports · 2026Article
- Genome Editing in the Chicken: From PGC-Mediated Germline Transmission to Advanced Applications.International journal of molecular sciences · 2025Review
- Improved surrogate eggshell incubation system for assisted reproductive technologies such as cloning, genome modification, and de-extinction efforts.Scientific reports · 2024Article
- Genetically modified chickens as bioreactors for protein-based drugs.Frontiers in genome editing · 2024Review
- Study of the regulatory elements of the Ovalbumin gene promoter using CRISPR technology in chicken cells.Journal of biological engineering · 2023Article
- Alterations in Immune-Related Defensin Alpha 4 (International journal of inflammation · 2022Review
- Isolation and characterization of cultured chicken oviduct epithelial cells and in vitro validation of constructed ovalbumin promoter in these cells.Animal bioscience · 2021Article
- Improving germline transmission efficiency in chimeric chickens using a multi-stage injection approach.PloS one · 2021Article
- Production of Recombinant Monoclonal Antibodies in the Egg White of Gene-Targeted Transgenic Chickens.Genes · 2020Article
- Designing A Transgenic Chicken: Applying New Approaches toward A Promising Bioreactor.Cell journal · 2020Article
- Current Approaches and Applications in Avian Genome Editing.International journal of molecular sciences · 2020Review
- Targeting lentiviral vectors to primordial germ cells (PGCs): An efficient strategy for generating transgenic chickens.Zoological research · 2020Article
- CRISPR/Cas9 gene editing in a chicken model: current approaches and applications.Journal of applied genetics · 2020Review
- Direct delivery of adenoviral CRISPR/Cas9 vector into the blastoderm for generation of targeted gene knockout in quail.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Avian Bioreactor Systems: A Review.Molecular biotechnology · 2018Review
- Efficient production of human interferon beta in the white of eggs from ovalbumin gene-targeted hens.Scientific reports · 2018Article
- Generation of transgenic chickens by the non-viral, cell-based method: effectiveness of some elements of this strategy.Journal of applied genetics · 2018Review
- Gene editing in birds takes flight.Mammalian genome : official journal of the International Mammalian Genome Society · 2017Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The expression of oviduct-specific recombinant proteins in transgenic chickens is a promising technology for the production of therapeutic biologics in eggs. In this study, we constructed a lentiviral vector encoding an expression cassette for human neutrophil defensin 4 (HNP4), a compound that displays high activity against Escherichia coli, and produced transgenic chickens that expressed the recombinant HNP4 protein in egg whites. After the antimicrobial activity of the recombinant HNP4 protein was tested at the cellular level, a 2.8-kb ovalbumin promoter was used to drive HNP4 expression specifically in oviduct tissues. From 669 injected eggs, 218 chickens were successfully hatched. Ten G0 roosters, with semens identified as positive for the transgene, were mated with wild-type hens to generate G1 chickens. From 1,274 total offspring, fifteen G1 transgenic chickens were positive for the transgene, which was confirmed by PCR and Southern blotting. The results of the Southern blotting and genome walking indicated that a single copy of the HNP4 gene was integrated into chromosomes 1, 2, 3, 4, 6 and 24 of the chickens. As expected, HNP4 expression was restricted to the oviduct tissues, and the levels of both transcriptional and translational HNP4 expression varied greatly in transgenic chickens with different transgene insertion sites. The amount of HNP4 protein expressed in the eggs of G1 and G2 heterozygous transgenic chickens ranged from 1.65 μg/ml to 10.18 μg/ml. These results indicated that the production of transgenic chickens that expressed HNP4 protein in egg whites was successful.
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