ArticlePLoS pathogens2015
Phosphatidic acid produced by phospholipase D promotes RNA replication of a plant RNA virus.
Article in PLoS pathogens, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 47 citations in OpenAlex.
- Activation of MAPK-mediated immunity by phosphatidic acid in response to positive-strand RNA viruses.Plant communications · 2024Article
- Manipulation of the Cellular Membrane-Cytoskeleton Network for RNA Virus Replication and Movement in Plants.Viruses · 2023Review
- A sword or a buffet: plant endomembrane system in viral infections.Frontiers in plant science · 2023Review
- Effects of the Noncoding Subgenomic RNA of Red Clover Necrotic Mosaic Virus in Virus Infection.Journal of virology · 2022Article
- Phosphatidic acid modulates MPK3- and MPK6-mediated hypoxia signaling in Arabidopsis.The Plant cell · 2022Article
- Phospholipases C and D and Their Role in Biotic and Abiotic Stresses.Plants (Basel, Switzerland) · 2021Review
- An engineered mutant of a host phospholipid synthesis gene inhibits viral replication without compromising host fitness.The Journal of biological chemistry · 2019Article
- Phytophthora infestans small phospholipase D-like proteins elicit plant cell death and promote virulence.Molecular plant pathology · 2019Article
- Host Lipids in Positive-Strand RNA Virus Genome Replication.Frontiers in microbiology · 2019Review
- Plant Hormone Signaling Crosstalks between Biotic and Abiotic Stress Responses.International journal of molecular sciences · 2018Review
- Host Pah1p phosphatidate phosphatase limits viral replication by regulating phospholipid synthesis.PLoS pathogens · 2018Article
- Three-Dimensional Architecture and Biogenesis of Membrane Structures Associated with Plant Virus Replication.Frontiers in plant science · 2018Review
- Roles of superoxide anion and hydrogen peroxide during replication of two unrelated plant RNA viruses in Nicotiana benthamiana.Plant signaling & behavior · 2017Article
- Targeting phospholipase D in cancer, infection and neurodegenerative disorders.Nature reviews. Drug discovery · 2017Review
- Harnessing host ROS-generating machinery for the robust genome replication of a plant RNA virus.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Diacylglycerol Kinases Are Widespread in Higher Plants and Display Inducible Gene Expression in Response to Beneficial Elements, Metal, and Metalloid Ions.Frontiers in plant science · 2017Article
- Dynamic lipid landscape of picornavirus replication organelles.Current opinion in virology · 2016Review
- Review
- Positive-strand RNA viruses stimulate host phosphatidylcholine synthesis at viral replication sites.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Eukaryotic positive-strand RNA [(+)RNA] viruses are intracellular obligate parasites replicate using the membrane-bound replicase complexes that contain multiple viral and host components. To replicate, (+)RNA viruses exploit host resources and modify host metabolism and membrane organization. Phospholipase D (PLD) is a phosphatidylcholine- and phosphatidylethanolamine-hydrolyzing enzyme that catalyzes the production of phosphatidic acid (PA), a lipid second messenger that modulates diverse intracellular signaling in various organisms. PA is normally present in small amounts (less than 1% of total phospholipids), but rapidly and transiently accumulates in lipid bilayers in response to different environmental cues such as biotic and abiotic stresses in plants. However, the precise functions of PLD and PA remain unknown. Here, we report the roles of PLD and PA in genomic RNA replication of a plant (+)RNA virus, Red clover necrotic mosaic virus (RCNMV). We found that RCNMV RNA replication complexes formed in Nicotiana benthamiana contained PLDα and PLDβ. Gene-silencing and pharmacological inhibition approaches showed that PLDs and PLDs-derived PA are required for viral RNA replication. Consistent with this, exogenous application of PA enhanced viral RNA replication in plant cells and plant-derived cell-free extracts. We also found that a viral auxiliary replication protein bound to PA in vitro, and that the amount of PA increased in RCNMV-infected plant leaves. Together, our findings suggest that RCNMV hijacks host PA-producing enzymes to replicate.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.