ArticleBiotechnology, biotechnological equipment2015
An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes.
Article in Biotechnology, biotechnological equipment, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 267 papers.
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267 citing papers in PubMed.
- A dual-functional dendrimer-based system integrating recombinant endolysin and cinnamic acid for enhanced endolysin stability and antibacterial activity against Acinetobacter baumannii.World journal of microbiology & biotechnology · 2026Article
- Efficient enzyme-mediated degradation of various emerging pollutants, including 2-mercaptobenzothiazole, paracetamol, and sulfamethoxazole, using HRP@UiO-66-NHEnvironmental science and pollution research international · 2026Article
- Magnetic Poly(VPA-co-EGDMA)-TiOPolymers · 2026Article
- Enzyme Co-Immobilization on Precipitated Silica for Sustainable Lactobionic Acid Production.Molecules (Basel, Switzerland) · 2026Article
- Sustainable biodiesel from an invasive fish using immobilized lipase on magnetic nanogels as a novel management approach.Scientific reports · 2026Article
- Immobilization of an Invertase Produced by a Bacterium Isolated from the Peach Palm Fruit (Journal of agricultural and food chemistry · 2026Article
- Going full circle: dynamic covalent enzyme immobilisationChemical science · 2026Article
- Article
- Advancing non-faradaic impedance biosensors: sensitivity enhancement strategies using microfluidics, multiscale labeling, and CMOS technology.Microsystems & nanoengineering · 2026Review
- Biosensor-Integrated Microneedle Devices for Diagnosis and Treatment of Chronic and Infectious Diseases: Current Status, Trends and Challenges.Biosensors · 2026Review
- Synthesis and characterization of ZrFeNanoscale advances · 2026Article
- Small buds to blooming flowers: organic-inorganic hybrid nanoflowers as immobilization platform and robust system for bio-catalytic production of d-allose.RSC advances · 2025Article
- Green COScientific reports · 2025Article
- Bovine Serum Albumin-Trypsin Sponges for Enhanced Enzymatic Stability and Protein Digestion Efficiency.ACS applied bio materials · 2025Article
- Prospects and problems in enzyme immobilization methodology: comprehensive review.Biophysical reviews · 2025Review
- Enhancing the Stability of Fungal Lipases by Immobilization onto Accurel MP 1000 Support and Additional Glutaraldehyde Crosslinking.Biomolecules · 2025Article
- Effect of pH and Particle Charge on the Interfacial Properties of Biocatalytic Pickering Emulsions─Where Are the Enzymes Located?Langmuir : the ACS journal of surfaces and colloids · 2025Article
- Graphitic Carbon Nitride: A Reusable and Stable Support for Glucose Oxidase.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- Advancements in the Research on the Preparation of Isoamyl Acetate Catalyzed by Immobilized Lipase.Materials (Basel, Switzerland) · 2025Review
- Preparation of immobilized xanthine oxidase with magnetic metal-organic framework and its application in screening of active ingredients in traditional Chinese medicine.Mikrochimica acta · 2025Article
207 more citing papers are in PubMed but not listed here.
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5 authors.
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Abstract
The current demands of sustainable green methodologies have increased the use of enzymatic technology in industrial processes. Employment of enzyme as biocatalysts offers the benefits of mild reaction conditions, biodegradability and catalytic efficiency. The harsh conditions of industrial processes, however, increase propensity of enzyme destabilization, shortening their industrial lifespan. Consequently, the technology of enzyme immobilization provides an effective means to circumvent these concerns by enhancing enzyme catalytic properties and also simplify downstream processing and improve operational stability. There are several techniques used to immobilize the enzymes onto supports which range from reversible physical adsorption and ionic linkages, to the irreversible stable covalent bonds. Such techniques produce immobilized enzymes of varying stability due to changes in the surface microenvironment and degree of multipoint attachment. Hence, it is mandatory to obtain information about the structure of the enzyme protein following interaction with the support surface as well as interactions of the enzymes with other proteins. Characterization technologies at the nanoscale level to study enzymes immobilized on surfaces are crucial to obtain valuable qualitative and quantitative information, including morphological visualization of the immobilized enzymes. These technologies are pertinent to assess efficacy of an immobilization technique and development of future enzyme immobilization strategies.
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