ArticleBiotechnology, biotechnological equipment2014
Molecular detection of bacteria in plant tissues, using universal 16S ribosomal DNA degenerated primers.
Article in Biotechnology, biotechnological equipment, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Host-selective PMA-PCR enhances bacterial diversity recovery and lowers detection limits in plant microbiome profiling.FEMS microbiology ecology · 2026Article
- Isolation, Identification and Pollution Prevention of Bacteria and Fungi during the Tissue Culture of Dwarf Hygro (Microorganisms · 2022Article
- Article
- Contamination detection in genomic data: more is not enough.Genome biology · 2022Review
- Molecular identification and biological control ofSaudi journal of biological sciences · 2021Article
- Isolation and Characterization of a Plant Growth-Promoting Bacterium Acinetobacter sp. SuKIC24 From in vitro-Grown Basilicum polystachyon (L.) Moench.Current microbiology · 2021Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Highly specific, sensitive and rapid tests are required for the detection and identification of covert bacterial contaminations in plant tissue cultures. Current methods available for this purpose are tedious, time consuming, highly error prone, expensive, require advanced technical expertise and are sometimes ineffective. We report here the development of a sensitive polymerase chain reaction (PCR) based method for the rapid detection and identification of bacteria occurring in plant tissue cultures. A total of 121 16S ribosomal DNA (rDNA) coding regions from 14 different groups of bacteria, algae and plants, available in the
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