Evidence map›Paper›PMID 26019210›Full record

ArticleJournal of clinical microbiology2015

Development and Evaluation of Novel Real-Time Reverse Transcription-PCR Assays with Locked Nucleic Acid Probes Targeting Leader Sequences of Human-Pathogenic Coronaviruses.

Jasper Fuk-Woo Chan, Garnet Kwan-Yue Choi, Alan Ka-Lun Tsang, Kah-Meng Tee, Ho-Yin Lam, Cyril Chik-Yan Yip, Kelvin Kai-Wang To, Vincent Chi-Chung Cheng, Man-Lung Yeung, Susanna Kar-Pui Lau and 4 more

Abstract readComparative StudyEvaluation Study
In one paragraph

Article in Journal of clinical microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.

0numbers the graph read from it
0cells of the map it votes in
53citing papers in PubMed
3.3field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

53 citing papers in PubMed, 89 citations in OpenAlex.

  1. Article
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  9. Mapping the footprints of COVID-19 pandemic.Journal of family medicine and primary care · 2021
    Review
  10. Review
  11. Article
  12. Current diagnostic and therapeutic strategies for COVID-19.Journal of pharmaceutical analysis · 2021
    Review
  13. Laboratory detection methods for the human coronaviruses.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2021
    Review
  14. Review
  15. MERS-CoV: epidemiology, molecular dynamics, therapeutics, and future challenges.Annals of clinical microbiology and antimicrobials · 2021
    Review
  16. Review
  17. Current Challenges for the Effective Management of the COVID-19 Pandemic.Advances in experimental medicine and biology · 2021
    Article
  18. Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 2 institutions in 1 country.

Jasper Fuk-Woo ChanState Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.
Garnet Kwan-Yue ChoiDepartment of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.
Alan Ka-Lun TsangDepartment of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.
Kah-Meng TeeDepartment of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.
Ho-Yin LamDepartment of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong Special Administrative Region, China.
Cyril Chik-Yan YipDepartment of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.
Kelvin Kai-Wang ToState Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.
Vincent Chi-Chung ChengDepartment of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.
Man-Lung YeungDepartment of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.
Susanna Kar-Pui LauState Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.
Patrick Chiu-Yat WooState Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.
Kwok-Hung ChanDepartment of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.
Bone Siu-Fai TangDepartment of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong Special Administrative Region, China.
Kwok-Yung YuenState Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China kyyuen@hku.hk.
University of Hong Kong · HKHong Kong Sanatorium and Hospital · HK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Based on findings in small RNA-sequencing (Seq) data analysis, we developed highly sensitive and specific real-time reverse transcription (RT)-PCR assays with locked nucleic acid probes targeting the abundantly expressed leader sequences of Middle East respiratory syndrome coronavirus (MERS-CoV) and other human coronaviruses. Analytical and clinical evaluations showed their noninferiority to a commercial multiplex PCR test for the detection of these coronaviruses.

Indexed as

AdolescentAdultAgedAged, 80 and overAnimalsChildChild, PreschoolCoronavirusCoronavirus InfectionsFemaleHumansInfantMaleMiddle AgedOligonucleotide ProbesOligonucleotideslocked nucleic acidOligonucleotide ProbesOligonucleotides

Identifiers

PMID26019210
PMCPMC4508434
OpenAlexW1568152060

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.