ArticleMolecular & cellular proteomics : MCP2015
The Human Pathogen Streptococcus pyogenes Releases Lipoproteins as Lipoprotein-rich Membrane Vesicles.
Article in Molecular & cellular proteomics : MCP, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 57 citations in OpenAlex.
- Identification of immunostimulatory antigens in Group AProceedings of the National Academy of Sciences of the United States of America · 2026Article
- From biogenesis to host modulation: the expanding biology of oral streptococcal membrane vesicles.FEMS microbiology reviews · 2026Review
- Streptococcus pyogenes EVs induce the alternative inflammasome via caspase-4/-5 in human monocytes.EMBO reports · 2025Article
- Standardizing Bacterial Extracellular Vesicle Purification: A Call for Consensus.Journal of microbiology and biotechnology · 2025Review
- Impact of antibiotics on membrane vesicle production in Group BMicrobiology spectrum · 2025Article
- Interactions of Gram-Positive Bacterial Membrane Vesicles and Hosts: Updates and Future Directions.International journal of molecular sciences · 2024Review
- Methods for Studying Fusion of Bacterial Extracellular Vesicles with Intact Bacteria and Host Cells.Methods in molecular biology (Clifton, N.J.) · 2024Article
- RopB-regulated SpeB cysteine protease degrades extracellular vesicles-associated streptolysin O and bacterial proteins from group AVirulence · 2023Article
- The Two Faces of Bacterial Membrane Vesicles: Pathophysiological Roles and Therapeutic Opportunities.Antibiotics (Basel, Switzerland) · 2023Review
- An update on our understanding of Gram-positive bacterial membrane vesicles: discovery, functions, and applications.Frontiers in cellular and infection microbiology · 2023Review
- Microbe-host interactions: structure and functions of Gram-negative bacterial membrane vesicles.Frontiers in microbiology · 2023Review
- Research Progress on Bacterial Membrane Vesicles and Antibiotic Resistance.International journal of molecular sciences · 2022Review
- Evolution of Streptococcus pyogenes has maximized the efficiency of the Sortase A cleavage motif for cell wall transpeptidation.The Journal of biological chemistry · 2022Article
- Extracellular vesicle formation in Lactococcus lactis is stimulated by prophage-encoded holin-lysin system.Microbial biotechnology · 2022Article
- Extracellular vesicles in the context of Mycobacterium tuberculosis infection.Molecular immunology · 2021Review
- Production of Membrane Vesicles inGenes · 2021Article
- Characterization and function of membrane vesicles in Gram-positive bacteria.Applied microbiology and biotechnology · 2021Review
- The composition and function ofmicroLife · 2021Article
- Biological Effect ofFrontiers in cellular and infection microbiology · 2021Article
- Significant increase in the secretion of extracellular vesicles and antibiotics resistance from methicillin-resistant Staphylococcus aureus induced by ampicillin stress.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
18 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial lipoproteins are attractive vaccine candidates because they represent a major class of cell surface-exposed proteins in many bacteria and are considered as potential pathogen-associated molecular patterns sensed by Toll-like receptors with built-in adjuvanticity. Although Gram-negative lipoproteins have been extensively characterized, little is known about Gram-positive lipoproteins. We isolated from Streptococcus pyogenes a large amount of lipoproteins organized in vesicles. These vesicles were obtained by weakening the bacterial cell wall with a sublethal concentration of penicillin. Lipid and proteomic analysis of the vesicles revealed that they were enriched in phosphatidylglycerol and almost exclusively composed of lipoproteins. In association with lipoproteins, a few hypothetical proteins, penicillin-binding proteins, and several members of the ExPortal, a membrane microdomain responsible for the maturation of secreted proteins, were identified. The typical lipidic moiety was apparently not necessary for lipoprotein insertion in the vesicle bilayer because they were also recovered from the isogenic diacylglyceryl transferase deletion mutant. The vesicles were not able to activate specific Toll-like receptor 2, indicating that lipoproteins organized in these vesicular structures do not act as pathogen-associated molecular patterns. In light of these findings, we propose to name these new structures Lipoprotein-rich Membrane Vesicles.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.