ArticleJournal of applied oral science : revista FOB
Expression of osteoblastic phenotype in periodontal ligament fibroblasts cultured in three-dimensional collagen gel.
Article in Journal of applied oral science : revista FOB. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 28 citations in OpenAlex.
- Osteogenic differentiation of gingival and periodontal ligament fibroblasts inhibits osteoclast formation in 2D and 3D fibrin cultures.Bone reports · 2026Article
- Periodontium-derived fibroblasts as a model to evaluate inflammation and pharmacological modulation of osteogenesis and osteoclast formation.Frontiers in cell and developmental biology · 2026Review
- Breaking Barriers: Evaluating Challenges in Advancing Periodontal Ligament Cell-Derived Organoids.Dentistry journal · 2025Review
- The profiling and analysis of gene expression in human periodontal ligament tissue and fibroblasts.Clinical and experimental dental research · 2022Article
- Comparison of Osteoblastic Differentiation of Human Periodontal Ligament Stem Cells through Application of Two β-tricalcium Phosphate Products: AnJournal of dentistry (Shiraz, Iran) · 2022Article
- Current Trends in In Vitro Modeling to Mimic Cellular Crosstalk in Periodontal Tissue.Advanced healthcare materials · 2021Review
- Distinguish fatty acids impact survival, differentiation and cellular function of periodontal ligament fibroblasts.Scientific reports · 2020Article
- RANKL deletion in periodontal ligament and bone lining cells blocks orthodontic tooth movement.International journal of oral science · 2018Article
- Three-dimensional printing for craniomaxillofacial regeneration.Journal of the Korean Association of Oral and Maxillofacial Surgeons · 2017Review
- Directed Differentiation of Human Embryonic Stem Cells Toward Placode-Derived Spiral Ganglion-Like Sensory Neurons.Stem cells translational medicine · 2017Article
- Influence of Titanium Surface Treatments on Viability of Periodontal Fibroblasts Grown in an Osteogenic Culture Medium.Contemporary clinical dentistryArticle
- Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathway.Journal of applied oral science : revista FOBArticle
- LiveIn vivo (Athens, Greece)Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo investigate the influence of a three-dimensional cell culture model on the expression of osteoblastic phenotype in human periodontal ligament fibroblast (hPDLF) cultures. MATERIAL AND
methodshPDLF were seeded on bi-dimensional (2D) and three-dimensional (3D) collagen type I (experimental groups) and and on a plastic coverslip (control) for up to 14 days. Cell viability and alkaline phosphatase (ALP) activity were performed. Also, cell morphology and immunolabeling for alkaline phosphatase (ALP) and osteopontin (OPN) were assessed by epifluorescence and confocal microscopy. The expression of osteogenic markers, including alkaline phosphatase, osteopontin, osteocalcin (OC), collagen I (COL I) and runt-related transcription factor 2 (RUNX2), were analyzed using real-time polymerase chain reaction (RT-PCR). Mineralized bone-like nodule formation was visualized by microscopy and calcium content was assessed quantitatively by alizarin red assay.
resultsExperimental cultures produced an increase in cell proliferation. Immunolabeling for OPN and ALP in hPDLF were increased and ALP activity was inhibited by three-dimensional conditions. OPN and RUNX2 gene expression was significantly higher on 3D culture when compared with control surface. Moreover, ALP and COL I gene expression were significantly higher in three-dimensional collagen than in 2D cultures at 7 days. However, at 14 days, 3D cultures exhibited ALP and COL I gene expression significantly lower than the control, and the COL I gene expression was also significantly lower in 3D than in 2D cultures. Significant calcium mineralization was detected and quantified by alizarin red assay, and calcified nodule formation was not affected by tridimensionality.
conclusionThis study suggests that the 3D cultures are able to support hPDLF proliferation and favor the differentiation and mineralized matrix formation, which may be a potential periodontal regenerative therapy.
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