Evidence map›Paper›PMID 26018250›Full record

ArticleEndocrinology2015

Testing the Critical Window Hypothesis of Timing and Duration of Estradiol Treatment on Hypothalamic Gene Networks in Reproductively Mature and Aging Female Rats.

Weiling Yin, Sean M Maguire, Brian Pham, Alexandra N Garcia, Nguyen-Vy Dang, Jingya Liang, Andrew Wolfe, Hans A Hofmann, Andrea C Gore

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Endocrinology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
1.6field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 25 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Weiling YinDivision of Pharmacology and Toxicology (W.Y., B.P., N.-V.D., J.L., A.C.G.), Departments of Integrative Biology (S.M.M., H.A.H.) and Psychology (A.N.G., A.C.G.), and Institute for Neuroscience (H.A.H., A.C.G.), The University of Texas at Austin, Austin, Texas 78712; and Johns Hopkins University School of Medicine (A.W.), Baltimore, Maryland 21287.
Sean M MaguireDivision of Pharmacology and Toxicology (W.Y., B.P., N.-V.D., J.L., A.C.G.), Departments of Integrative Biology (S.M.M., H.A.H.) and Psychology (A.N.G., A.C.G.), and Institute for Neuroscience (H.A.H., A.C.G.), The University of Texas at Austin, Austin, Texas 78712; and Johns Hopkins University School of Medicine (A.W.), Baltimore, Maryland 21287.
Brian PhamDivision of Pharmacology and Toxicology (W.Y., B.P., N.-V.D., J.L., A.C.G.), Departments of Integrative Biology (S.M.M., H.A.H.) and Psychology (A.N.G., A.C.G.), and Institute for Neuroscience (H.A.H., A.C.G.), The University of Texas at Austin, Austin, Texas 78712; and Johns Hopkins University School of Medicine (A.W.), Baltimore, Maryland 21287.
Alexandra N GarciaDivision of Pharmacology and Toxicology (W.Y., B.P., N.-V.D., J.L., A.C.G.), Departments of Integrative Biology (S.M.M., H.A.H.) and Psychology (A.N.G., A.C.G.), and Institute for Neuroscience (H.A.H., A.C.G.), The University of Texas at Austin, Austin, Texas 78712; and Johns Hopkins University School of Medicine (A.W.), Baltimore, Maryland 21287.
Nguyen-Vy DangDivision of Pharmacology and Toxicology (W.Y., B.P., N.-V.D., J.L., A.C.G.), Departments of Integrative Biology (S.M.M., H.A.H.) and Psychology (A.N.G., A.C.G.), and Institute for Neuroscience (H.A.H., A.C.G.), The University of Texas at Austin, Austin, Texas 78712; and Johns Hopkins University School of Medicine (A.W.), Baltimore, Maryland 21287.
Jingya LiangDivision of Pharmacology and Toxicology (W.Y., B.P., N.-V.D., J.L., A.C.G.), Departments of Integrative Biology (S.M.M., H.A.H.) and Psychology (A.N.G., A.C.G.), and Institute for Neuroscience (H.A.H., A.C.G.), The University of Texas at Austin, Austin, Texas 78712; and Johns Hopkins University School of Medicine (A.W.), Baltimore, Maryland 21287.
Andrew WolfeDivision of Pharmacology and Toxicology (W.Y., B.P., N.-V.D., J.L., A.C.G.), Departments of Integrative Biology (S.M.M., H.A.H.) and Psychology (A.N.G., A.C.G.), and Institute for Neuroscience (H.A.H., A.C.G.), The University of Texas at Austin, Austin, Texas 78712; and Johns Hopkins University School of Medicine (A.W.), Baltimore, Maryland 21287.
Hans A HofmannDivision of Pharmacology and Toxicology (W.Y., B.P., N.-V.D., J.L., A.C.G.), Departments of Integrative Biology (S.M.M., H.A.H.) and Psychology (A.N.G., A.C.G.), and Institute for Neuroscience (H.A.H., A.C.G.), The University of Texas at Austin, Austin, Texas 78712; and Johns Hopkins University School of Medicine (A.W.), Baltimore, Maryland 21287.
Andrea C GoreDivision of Pharmacology and Toxicology (W.Y., B.P., N.-V.D., J.L., A.C.G.), Departments of Integrative Biology (S.M.M., H.A.H.) and Psychology (A.N.G., A.C.G.), and Institute for Neuroscience (H.A.H., A.C.G.), The University of Texas at Austin, Austin, Texas 78712; and Johns Hopkins University School of Medicine (A.W.), Baltimore, Maryland 21287.
The University of Texas at Austin · USJohns Hopkins Medicine · US

Funding

The Nonhuman Primate CoreP01AG016765 · NIA · MOUNT SINAI SCHOOL OF MEDICINE OF NYU · PI BAXTER, MARK G · 1999 to 2016
$26.1M
JHU-UMD Diabetes Research CenterP30DK079637 · NIDDK · JOHNS HOPKINS UNIVERSITY · PI AHIMA, REXFORD S. · 2013 to 2017
$9.7M
Diabetes Research and Training CenterP60DK079637 · NIDDK · JOHNS HOPKINS UNIVERSITY · PI WONDISFORD, FREDRIC E. · 2008 to 2012
$9.1M
NIA NIH HHS AG16765NIA NIH HHS P01 AG016765NIDDK NIH HHS P60 DK079637
6 · The paper itself

Abstract

At menopause, the dramatic loss of ovarian estradiol (E2) necessitates the adaptation of estrogen-sensitive neurons in the hypothalamus to an estrogen-depleted environment. We developed a rat model to test the "critical window" hypothesis of the effects of timing and duration of E2 treatment after deprivation on the hypothalamic neuronal gene network in the arcuate nucleus and the medial preoptic area. Rats at 2 ages (reproductively mature or aging) were ovariectomized and given E2 or vehicle replacement regimes of differing timing and duration. Using a 48-gene quantitative low-density PCR array and weighted gene coexpression network analysis, we identified gene modules differentially regulated by age, timing, and duration of E2 treatment. Of particular interest, E2 status differentially affected suites of genes in the hypothalamus involved in energy balance, circadian rhythms, and reproduction. In fact, E2 status was the dominant factor in determining gene modules and hormone levels; age, timing, and duration had more subtle effects. Our results highlight the plasticity of hypothalamic neuroendocrine systems during reproductive aging and its surprising ability to adapt to diverse E2 replacement regimes.

Indexed as

ReproductionSexual MaturationAgingAnimalsEstradiolFemaleGene Regulatory NetworksHypothalamusModels, BiologicalRatsRats, Sprague-DawleyTime FactorsEstradiol

Identifiers

PMID26018250
PMCPMC4511137
OpenAlexW1956397921

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.