ArticlePloS one2015
Characterization and PCR Detection Of Binary, Pir-Like Toxins from Vibrio parahaemolyticus Isolates that Cause Acute Hepatopancreatic Necrosis Disease (AHPND) in Shrimp.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed.
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- NativePathogens (Basel, Switzerland) · 2026Article
- Excessive activation of JAK-STAT signaling contributes to inflammation induced by acuteVirulence · 2025Article
- History of Shrimp Farming and the Main Viral and Bacterial Diseases in Mexico.Microorganisms · 2025Review
- Novel midgut smooth muscle necrosis (MSMN) in translucent or glass post-larvae of whiteleg shrimp.Scientific reports · 2025Article
- Biofloc system with synbiotic supplementation improves Penaeus vannamei immunity against AHPND.Applied microbiology and biotechnology · 2025Article
- Report on Vibrio Species Contamination in Shrimp From the Coast of Pangandaran, West Java, Indonesia.Environmental microbiology reports · 2025Article
- Complete genome sequences ofData in brief · 2025Article
- Host genome drives the microbiota enrichment of beneficial microbes in shrimp: exploring the hologenome perspective.Animal microbiome · 2025Article
- Article
- Genomic and Pathological Characterization of Acute Hepatopancreatic Necrosis Disease (AHPND)-Associated Natural MutantAnimals : an open access journal from MDPI · 2024Article
- Review
- The effects ofFish and shellfish immunology reports · 2023Article
- Article
- Genomic and Evolutionary Features of Nine AHPND Positive Vibrio parahaemolyticus Strains Isolated from South American Shrimp Farms.Microbiology spectrum · 2023Article
- Novel sandwich immunoassay detects a shrimp AHPND-causing binary PirABFrontiers in cellular and infection microbiology · 2023Article
- Signature selection forces and evolutionary divergence of immune-survival genes compared between two important shrimp species.PloS one · 2023Article
- Fabrication of lateral flow immunoassay strip for rapid detection of acute hepatopancreatic necrosis disease.3 Biotech · 2022Article
- Biochemical indexes and gut microbiota testing as diagnostic methods forFood science & nutrition · 2022Article
- Supplementation of ex situ produced bioflocs improves immune response against AHPND in Pacific whiteleg shrimp (Litopenaeus vannamei) postlarvae.Applied microbiology and biotechnology · 2022Article
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10 authors.
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Abstract
Unique isolates of Vibrio parahaemolyticus (VPAHPND) have previously been identified as the causative agent of acute hepatopancreatic necrosis disease (AHPND) in shrimp. AHPND is characterized by massive sloughing of tubule epithelial cells of the hepatopancreas (HP), proposed to be induced by soluble toxins released from VPAHPND that colonize the shrimp stomach. Since these toxins (produced in broth culture) have been reported to cause AHPND pathology in reverse gavage bioassays with shrimp, we used ammonium sulfate precipitation to prepare protein fractions from broth cultures of VPAHPND isolates for screening by reverse gavage assays. The dialyzed 60% ammonium sulfate fraction caused high mortality within 24-48 hours post-administration, and histological analysis of the moribund shrimp showed typical massive sloughing of hepatopancreatic tubule epithelial cells characteristic of AHPND. Analysis of the active fraction by SDS-PAGE revealed two major bands at marker levels of approximately 16 kDa (ToxA) and 50 kDa (ToxB). Mass spectrometry analysis followed by MASCOT analysis revealed that both proteins had similarity to hypothetical proteins of V. parahaemolyticus M0605 (contig034 GenBank accession no. JALL01000066.1) and similarity to known binary insecticidal toxins called 'Photorhabdus insect related' proteins A and B (Pir-A and Pir-B), respectively, produced by the symbiotic, nematode bacterium Photorhabdus luminescens. In in vivo tests, it was shown that recombinant ToxA and ToxB were both required in a dose dependent manner to cause AHPND pathology, indicating further similarity to Pir-A and -B. A single-step PCR method was designed for detection of the ToxA gene and was validated using 104 bacterial isolates consisting of 51 VPAHPND isolates, 34 non-AHPND VP isolates and 19 other isolates of bacteria commonly found in shrimp ponds (including other species of Vibrio and Photobacterium). The results showed 100% specificity and sensitivity for detection of VPAHPND isolates in the test set.
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