ArticleMolecular and cellular biology1989
The 216-nucleotide intron of the E1A pre-mRNA contains a hairpin structure that permits utilization of unusually distant branch acceptors.
Article in Molecular and cellular biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- A tailed mirtron promotes longevity in Drosophila.Nucleic acids research · 2024Article
- Exonic splicing code and coordination of divalent metals in proteins.Nucleic acids research · 2024Article
- Cooperative Binding of SRSF3 to Structured 3'ss-α Exon RNA during α Exon Inclusion in the ZO-1 mRNA.Current issues in molecular biology · 2023Article
- Restriction of an intron size en route to endothermy.Nucleic acids research · 2021Article
- Alternative splicing: Human disease and quantitative analysis from high-throughput sequencing.Computational and structural biotechnology journal · 2021Review
- Alternative splicing of U2AF1 reveals a shared repression mechanism for duplicated exons.Nucleic acids research · 2017Article
- HIV-1 Vpr N-terminal tagging affects alternative splicing of the viral genome.Scientific reports · 2016Article
- Diverse regulation of 3' splice site usage.Cellular and molecular life sciences : CMLS · 2015Review
- Role of RNA structure in regulating pre-mRNA splicing.Trends in biochemical sciences · 2010Review
- Modulating role of RNA structure in alternative splicing of a critical exon in the spinal muscular atrophy genes.Nucleic acids research · 2007Article
- Influence of RNA secondary structure on the pre-mRNA splicing process.Molecular and cellular biology · 2004Review
- A variable dinucleotide repeat in the CFTR gene contributes to phenotype diversity by forming RNA secondary structures that alter splicing.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Delineation of the mechanisms of aberrant splicing caused by two unusual intronic mutations in the RSK2 gene involved in Coffin-Lowry syndrome.Nucleic acids research · 2004Article
- A stem structure in fibroblast growth factor receptor 2 transcripts mediates cell-type-specific splicing by approximating intronic control elements.Molecular and cellular biology · 2003Article
- Conserved stem-loop structures in the HIV-1 RNA region containing the A3 3' splice site and its cis-regulatory element: possible involvement in RNA splicing.Nucleic acids research · 2001Article
- Characterization of human RNA splice signals by iterative functional selection of splice sites.RNA (New York, N.Y.) · 2000Article
- Identification of a bidirectional splicing enhancer: differential involvement of SR proteins in 5' or 3' splice site activation.Molecular and cellular biology · 1999Article
- Genetic studies exposing the splicing events involved in herpes simplex virus type 1 latency-associated transcript production during lytic and latent infection.Journal of virology · 1999Article
- Regulation of fibronectin EDA exon alternative splicing: possible role of RNA secondary structure for enhancer display.Molecular and cellular biology · 1999Article
- Multiple interdependent sequence elements control splicing of a fibroblast growth factor receptor 2 alternative exon.Molecular and cellular biology · 1997Article
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Authors and funding
5 authors.
Funding
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Abstract
A recently characterized 216-nucleotide intron-splicing reaction occurs within the adenovirus E1A pre-mRNA through the use of three branch acceptor sites, located at 59, 55, and 51 nucleotides from the 3' splice site. To investigate the role of the cis-acting sequence elements in the selection of such unusually distant branch sites, transcripts differing in sequence downstream of the branch sites were analyzed for in vitro splicing. Initial results suggested that secondary structure could be involved in the use of distant branch sites. The involvement of a hairpin structure, including a nine-G C-base-pair stem, was supported by the results of site-directed mutagenesis analyses. Mutations that destroyed or weakened this hairpin resulted in an inefficient splicing reaction. In contrast, complementary mutation or deletion of two bulges, which involved a restoration or reinforcement of the hairpin, resulted in a reactivation or improvement of the splicing efficiency, respectively. Therefore, we conclude that the hairpin structure shortens the operational distance between the 3' splice site and the branch acceptors and brings the branch sites into the branch-permissive window, 18 to 40 nucleotides upstream of the 3' splice site. Our results confirm the importance of the constraint of distance for the splicing reaction and show that this constraint may be overcome by means of a stable hairpin formation.
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