ArticlePathogens (Basel, Switzerland)2015
Development of a nucleic Acid extraction procedure for simultaneous recovery of DNA and RNA from diverse microbes in water.
Article in Pathogens (Basel, Switzerland), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 44 citations in OpenAlex.
- A Randomized Controlled Trial to Assess the Impact of Ceramic Water Filters on Prevention of Diarrhea and Cryptosporidiosis in Infants and Young Children-Western Kenya, 2013.The American journal of tropical medicine and hygiene · 2018Trial
- Large-volume sampling for improved characterization of microbial contamination in agricultural waters: a case study from southeastern U.S. produce farms.Journal of water and health · 2026Article
- Detection of Cryptosporidium Recovered from Large-Volume Water Samples Using Dead-End Ultrafiltration.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Single-Tube Capture, Concentration, and Genomic Extraction of a Human Norovirus Surrogate Using Magnetic Ionic Liquids.Analytical chemistry · 2025Article
- Evaluation of nucleic acid extraction methods for recovery of Cyclospora cayetanensis, Salmonella enterica, and murine norovirus from water and sludge.Journal of microbiological methods · 2025Article
- Performance of Conditional Random Forest and Regression Models at Predicting Human Fecal Contamination of Produce Irrigation Ponds in the Southeastern United States.ACS ES&T water · 2024Article
- Microbial Characterization, Factors Contributing to Contamination, and Household Use of Cistern Water, U.S. Virgin Islands.ACS ES&T water · 2022Article
- Detection ofMicroorganisms · 2022Review
- Use of guanidine thiocyanate-based nucleic acid extraction buffers to inactivate poliovirus in potentially infectious materials.Journal of virological methods · 2021Article
- Antimicrobial resistance genes are enriched in aerosols near impacted urban surface waters in La Paz, Bolivia.Environmental research · 2021Article
- Detection ofFood and waterborne parasitology · 2021Article
- Identifying Inorganic Turbidity in Water Samples as Potential Loss Factor During Nucleic Acid Extraction: Implications for Molecular Fecal Pollution Diagnostics and Source Tracking.Frontiers in microbiology · 2021Article
- Article
- Detection of Cryptosporidium Recovered from Large-Volume Water Samples Using Dead-End Ultrafiltration.Methods in molecular biology (Clifton, N.J.) · 2020Article
- A new solid matrix for preservation of viral nucleic acid from clinical specimens at ambient temperature.Journal of virological methods · 2019Article
- Two Drinking Water Outbreaks Caused by Wastewater Intrusion Including Sapovirus in Finland.International journal of environmental research and public health · 2019Article
- Response and remediation actions following the detection of Naegleria fowleri in two treated drinking water distribution systems, Louisiana, 2013-2014.Journal of water and health · 2019Article
- First Reported Human Cases of Leptospirosis in the United States Virgin Islands in the Aftermath of Hurricanes Irma and Maria, September-November 2017.Open forum infectious diseases · 2019Article
- Bioaerosol emissions associated with pit latrine emptying operations.The Science of the total environment · 2019Article
- All in One High Quality Genomic DNA and Total RNA Extraction From Nematode Induced Galls for High Throughput Sequencing Purposes.Frontiers in plant science · 2019Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Drinking and environmental water samples contain a diverse array of constituents that can interfere with molecular testing techniques, especially when large volumes of water are concentrated to the small volumes needed for effective molecular analysis. In this study, a suite of enteric viruses, bacteria, and protozoan parasites were seeded into concentrated source water and finished drinking water samples, in order to investigate the relative performance of nucleic acid extraction techniques for molecular testing. Real-time PCR and reverse transcription-PCR crossing threshold (CT) values were used as the metrics for evaluating relative performance. Experimental results were used to develop a guanidinium isothiocyanate-based lysis buffer (UNEX buffer) that enabled effective simultaneous extraction and recovery of DNA and RNA from the suite of study microbes. Procedures for bead beating, nucleic acid purification, and PCR facilitation were also developed and integrated in the protocol. The final lysis buffer and sample preparation procedure was found to be effective for a panel of drinking water and source water concentrates when compared to commercial nucleic acid extraction kits. The UNEX buffer-based extraction protocol enabled PCR detection of six study microbes, in 100 L finished water samples from four drinking water treatment facilities, within three CT values (i.e., within 90% difference) of the reagent-grade water control. The results from this study indicate that this newly formulated lysis buffer and sample preparation procedure can be useful for standardized molecular testing of drinking and environmental waters.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.