ArticleMolecular therapy. Methods & clinical development2014
Genetic rearrangements of variable di-residue (RVD)-containing repeat arrays in a baculoviral TALEN system.
Article in Molecular therapy. Methods & clinical development, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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Who cites it
10 citing papers in PubMed, 19 citations in OpenAlex.
- Progress and pitfalls of gene editing technology in CAR-T cell therapy: a state-of-the-art review.Frontiers in oncology · 2024Review
- Current State of Human Gene Therapy: Approved Products and Vectors.Pharmaceuticals (Basel, Switzerland) · 2023Review
- A CRISPR way for accelerating cereal crop improvement: Progress and challenges.Frontiers in genetics · 2022Review
- Therapeutic application of the CRISPR system: current issues and new prospects.Human genetics · 2019Review
- Exploiting the sequence diversity of TALE-like repeats to vary the strength of dTALE-promoter interactions.Synthetic biology (Oxford, England) · 2017Article
- Article
- DNA-binding proteins from marine bacteria expand the known sequence diversity of TALE-like repeats.Nucleic acids research · 2015Article
- Efficient delivery of nuclease proteins for genome editing in human stem cells and primary cells.Nature protocols · 2015Article
- Improved cell-penetrating zinc-finger nuclease proteins for precision genome engineering.Molecular therapy. Nucleic acids · 2015Article
- QueTAL: a suite of tools to classify and compare TAL effectors functionally and phylogenetically.Frontiers in plant science · 2015Article
Corrections and comments
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Authors and funding
16 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Virus-derived gene transfer vectors have been successfully employed to express the transcription activator-like effector nucleases (TALENs) in mammalian cells. Since the DNA-binding domains of TALENs consist of the variable di-residue (RVD)-containing tandem repeat modules and virus genome with repeated sequences is susceptible to genetic recombination, we investigated several factors that might affect TALEN cleavage efficiency of baculoviral vectors. Using a TALEN system designed to target the AAVS1 locus, we observed increased sequence instability of the TALE repeat arrays when a higher multiplicity of infection (MOI) of recombinant viruses was used to produce the baculoviral vectors. We also detected more deleterious mutations in the TALE DNA-binding domains when both left and right TALEN arms were placed into a single expression cassette as compared to the viruses containing one arm only. The DNA sequence changes in the domains included deletion, addition, substitution, and DNA strand exchange between the left and right TALEN arms. Based on these observations, we have developed a protocol using a low MOI to produce baculoviral vectors expressing TALEN left and right arms separately. Cotransduction of the viruses produced by this optimal protocol provided an improved TALEN cleavage efficiency and enabled effective site-specific transgene integration in human cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.