ArticleMolecular therapy. Methods & clinical development2014
Optimization of a gene electrotransfer procedure for efficient intradermal immunization with an hTERT-based DNA vaccine in mice.
Article in Molecular therapy. Methods & clinical development, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- Telomerase-based vaccines: a promising frontier in cancer immunotherapy.Cancer cell international · 2024Review
- Plasmid DNA Delivery into the Skin via Electroporation with a Depot-Type Electrode.Pharmaceutics · 2024Article
- Telomere-related DNA damage response pathways in cancer therapy: prospective targets.Frontiers in pharmacology · 2024Review
- Article
- Review
- Electroporation as the Immunotherapy Strategy for Cancer in Veterinary Medicine: State of the Art in Latin America.Vaccines · 2020Review
- Anti-cancer Immunotherapies Targeting Telomerase.Cancers · 2020Review
- A DNA telomerase vaccine for canine cancer immunotherapy.Oncotarget · 2019Article
- Numerical study of gene electrotransfer efficiency based on electroporation volume and electrophoretic movement of plasmid DNA.Biomedical engineering online · 2018Article
- Electrotransfer of Different Control Plasmids Elicits Different Antitumor Effectiveness in B16.F10 Melanoma.Cancers · 2018Article
- Upregulation of DNA Sensors in B16.F10 Melanoma Spheroid Cells After Electrotransfer of pDNA.Technology in cancer research & treatment · 2018Article
- Exploring the Applicability of Nano-Poration for Remote Control in Smart Drug Delivery Systems.The Journal of membrane biology · 2017Article
- Perspectives on Transdermal Electroporation.Pharmaceutics · 2016Review
- Anticancer DNA vaccine based on human telomerase reverse transcriptase generates a strong and specific T cell immune response.Oncoimmunology · 2016Article
- Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransfer.Cancer immunology, immunotherapy : CII · 2015Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA vaccination consists in administering an antigen-encoding plasmid in order to trigger a specific immune response. This specific vaccine strategy is of particular interest to fight against various infectious diseases and cancer. Gene electrotransfer is the most efficient and safest non-viral gene transfer procedure and specific electrical parameters have been developed for several target tissues. Here, a gene electrotransfer protocol into the skin has been optimized in mice for efficient intradermal immunization against the well-known telomerase tumor antigen. First, the luciferase reporter gene was used to evaluate gene electrotransfer efficiency into the skin as a function of the electrical parameters and electrodes, either non-invasive or invasive. In a second time, these parameters were tested for their potency to generate specific cellular CD8 immune responses against telomerase epitopes. These CD8 T-cells were fully functional as they secreted IFNγ and were endowed with specific cytotoxic activity towards target cells. This simple and optimized procedure for efficient gene electrotransfer into the skin using the telomerase antigen is to be used in cancer patients for the phase 1 clinical evaluation of a therapeutic cancer DNA vaccine called INVAC-1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.