ArticleAdvanced biomedical research2015
Use of restriction fragment length polymorphism to identify Candida species, related to onychomycosis.
Article in Advanced biomedical research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- Molecular identification and antifungal susceptibility profile of rare and emerging yeast species causing onychomycosis.BMC research notes · 2025Article
- Molecular identification of yeast communities isolated from nail specimens by PCR-RFLP and PCR-FSP methods.Current medical mycology · 2024Article
- Species distribution and antifungal susceptibility profiles of yeasts isolated from onychomycosis: a cross-sectional study with insights into emerging species.Antonie van Leeuwenhoek · 2023Article
- Antifungal Resistance of ClinicalIranian journal of public health · 2023Review
- Advances in Chemical and Biological Methods to Identify Microorganisms-From Past to Present.Microorganisms · 2019Review
- Microsatellite genotyping of clinical Candida parapsilosis isolates.Current medical mycology · 2017Article
- Comparison of CHROMagar, polymerase chain reaction-restriction fragment length polymorphism, and polymerase chain reaction-fragment size for the identification of Candida species.Current medical mycology · 2017Article
- Quantification of CDR1 Gene Expression in Fluconazole Resistant Candida Glabrata Strains Using Real-time PCR.Iranian journal of public health · 2017Article
- Fungal Biofilms and Polymicrobial Diseases.Journal of fungi (Basel, Switzerland) · 2017Review
- Caspofungin-Non-SusceptibleIranian journal of public health · 2017Article
- Causative Agents of Onychomycosis: A 7-Year Study.Journal of clinical laboratory analysis · 2016Article
- The identification and differentiation of the Candida parapsilosis complex species by polymerase chain reaction-restriction fragment length polymorphism of the internal transcribed spacer region of the rDNA.Memorias do Instituto Oswaldo Cruz · 2016Article
- Candida infections among neutropenic patients.Caspian journal of internal medicine · 2016Article
- Article
- Noisy neighbourhoods: quorum sensing in fungal-polymicrobial infections.Cellular microbiology · 2015Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOnychomycosis is one of the most common clinical forms of fungal infections due to both filamentous fungi and yeasts. The genus of Candida is one of the most prominent causes of onychomycosis in all around the world. Although Candida albicans is still the most frequent cause of nail infections, use of broad-spectrum antifungal agents has led to a shift in the etiology of C. albicans to non-albicans species. The aim of the present study is rapid and precise identification of candida species isolated from nail infection by using of PCR-RFLP technique. MATERIALS AND
methodsA total of 360 clinical yeast strains were collected from nail infections in Iran. Genomic DNA was extracted using FTA(;) cards. ITS1-5.8SrDNA-ITS2 region was amplified using universal primers and subsequently products were digested with the restriction enzyme MspI. For identification of newly described species (C. parapsilosis complex), the SADH gene was amplified, followed by digestion with Nla III restriction enzyme.
resultsCandida albicans was the most commonly isolated species (41.1%), followed by C. parapsilosis (21.4%), C. tropicalis (12.8%), C. kefyr (9.4%), C. krusei (5.5%), C. orthopsilosis (4.1%), C. glabrata (2.8%), C. guilliermondii (1.4%), C. rugosa (0.8%), and C. lusitaniae (0.5%). Patients in the age groups of 51-60 and 81-90 years had the highest and lowest distribution of positive specimens, respectively.
conclusionRapid and precise identification of Candida species from clinical specimens lead to appropriate therapeutic plans.
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