Evidence map›Paper›PMID 26015913›Full record

ArticleAdvanced biomedical research2015

Isolation of toxigenic Clostridium difficile from ready-to-eat salads by multiplex polymerase chain reaction in Isfahan, Iran.

Mahire Yamoudy, Maryam Mirlohi, Bahram Nasr Isfahani, Mohammad Jalali, Zahra Esfandiari, Nafiseh Sadat Hosseini

Abstract read
In one paragraph

Article in Advanced biomedical research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 2 syntheses or guidelines pooled it.

  1. Pooled it
  2. Global and Historical Distribution ofFrontiers in medicine · 2020
    Pooled it
  3. Review
  4. Review
  5. Detection and Genomic Characterisation ofPathogens (Basel, Switzerland) · 2022
    Article
  6. Genomic Delineation of Zoonotic Origins ofFrontiers in public health · 2019
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Mahire YamoudyDeparment of Food Science and Technology, Food Security Research Center, School of Nutrition and Food Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
Maryam MirlohiDeparment of Food Science and Technology, Food Security Research Center, School of Nutrition and Food Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
Bahram Nasr IsfahaniDeparment of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Mohammad JalaliDeparment of Food Science and Technology, Food Security Research Center, School of Nutrition and Food Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
Zahra EsfandiariDepartment of Research and Development, Deputy of Food and Drug, Isfahan University of Medical Sciences, Isfahan, Iran.
Nafiseh Sadat HosseiniDeparment of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundSince 2003, the incidence of community associated Clostridium difficile infection (CA-CDI) has increased; different types of food have been supposed to be the vectors of C. difficile strains. The purpose of this study is to investigate the occurrence of C. difficile strains in ready-to-eat salads distributed in food services. MATERIALS AND

methodsA total of 106 ready-made salad specimens were sampled from different restaurants and food services located in Isfahan, in the center of Iran. Positive isolates of C. difficile were identified and confirmed for the existence of three genes including tpi, tcdA and tcdB by multiplex PCR.

resultsA total of six (5.66%) samples were positive for C. difficile strains. Of which, one strain (16.6%) was positive for A and B toxins.

conclusionThe existence of toxigenic C. difficile in ready-made salads could be a caution for public health. Further investigation is required to assess the relationship between the isolated strains in our study and those from diarrheic patients through molecular typing.

Indexed as

Clostridium difficilemultiplex PCRprevalencesaladvegetable

Identifiers

PMID26015913
PMCPMC4434448

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.