ArticleProceedings of the National Academy of Sciences of the United States of America2015
Reduced endogenous Ca2+ buffering speeds active zone Ca2+ signaling.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
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- Phospholipid Scramblase 1 Controls Efficient Neurotransmission and Synaptic Vesicle Retrieval at Cerebellar Synapses.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Intracellular magnesium optimizes transmission efficiency and plasticity of hippocampal synapses by reconfiguring their connectivity.Nature communications · 2024Article
- A unified model for the dynamics of ATP-independent ultrafast contraction.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Calcium imaging and BAPTA loading of amygdala astrocytes in mouse brain slices.STAR protocols · 2022Article
- A theory of synaptic transmission.eLife · 2021Article
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- An on-demand, drop-on-drop method for studying enzyme catalysis by serial crystallography.Nature communications · 2021Article
- Separation of presynaptic CaProceedings of the National Academy of Sciences of the United States of America · 2021Article
- CaeLife · 2020Article
- HCN channel-mediated neuromodulation can control action potential velocity and fidelity in central axons.eLife · 2019Article
- EGTA Can Inhibit Vesicular Release in the Nanodomain of Single CaFrontiers in synaptic neuroscience · 2019Article
- Presynaptic Calcium En Passage through the Axon.Biophysical journal · 2018Article
- Diffusion of CaBiophysical journal · 2018Article
- Apparent calcium dependence of vesicle recruitment.The Journal of physiology · 2018Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fast synchronous neurotransmitter release at the presynaptic active zone is triggered by local Ca(2+) signals, which are confined in their spatiotemporal extent by endogenous Ca(2+) buffers. However, it remains elusive how rapid and reliable Ca(2+) signaling can be sustained during repetitive release. Here, we established quantitative two-photon Ca(2+) imaging in cerebellar mossy fiber boutons, which fire at exceptionally high rates. We show that endogenous fixed buffers have a surprisingly low Ca(2+)-binding ratio (∼ 15) and low affinity, whereas mobile buffers have high affinity. Experimentally constrained modeling revealed that the low endogenous buffering promotes fast clearance of Ca(2+) from the active zone during repetitive firing. Measuring Ca(2+) signals at different distances from active zones with ultra-high-resolution confirmed our model predictions. Our results lead to the concept that reduced Ca(2+) buffering enables fast active zone Ca(2+) signaling, suggesting that the strength of endogenous Ca(2+) buffering limits the rate of synchronous synaptic transmission.
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