ArticleProceedings of the National Academy of Sciences of the United States of America2015
Characterization of cooperative bicarbonate uptake into chloroplast stroma in the green alga Chlamydomonas reinhardtii.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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64 citing papers in PubMed, 142 citations in OpenAlex.
- Pyrenoid-based CO2-concentrating mechanisms across diverse lineages: convergent features and their applications for engineering.Plant physiology · 2026Review
- RBMP2 shapes specialized membranes for CObioRxiv : the preprint server for biology · 2026Article
- Architectural and biochemical advances in understanding microbial COThe Plant journal : for cell and molecular biology · 2026Review
- Time-dependent metabolic reprogramming in Scenedesmus sp. induced by intermittently sparged COScientific reports · 2026Article
- A nuclear CobW/WW-domain factor represses the COProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Metabolic rewiring and biomass redistribution enable optimized mixotrophic growth in Chlamydomonas.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Regulatory logic of theFrontiers in plant science · 2026Review
- Structure of Chlamydomonas reinhardtii LciA guided the engineering of FNT family proteins to gain bicarbonate transport activity.Nature plants · 2026Article
- Metabolic Engineering of Terpenoid Biosynthesis in Medicinal Plants: From Genomic Insights to Biotechnological Applications.Current issues in molecular biology · 2025Review
- The green algae CONature communications · 2025Article
- Periplasmic carbonic anhydrase CAH1 contributes to high inorganic carbon affinity in Chlamydomonas reinhardtii.Plant physiology · 2024Article
- Photosynthetic Electron Flows and Networks of Metabolite Trafficking to Sustain Metabolism in Photosynthetic Systems.Plants (Basel, Switzerland) · 2024Review
- Chloroplast biogenesis involves spatial coordination of nuclear and organellar gene expression in Chlamydomonas.Plant physiology · 2024Article
- Coordinated wound responses in a regenerative animal-algal holobiont.Nature communications · 2024Article
- The pyrenoid: the eukaryotic CO2-concentrating organelle.The Plant cell · 2023Article
- One step further toward a crop CO2-concentrating mechanism.Journal of experimental botany · 2023Article
- A pyrenoid-localized protein SAGA1 is necessary for CaPhotosynthesis research · 2023Article
- Adapting from Low to High: An Update to COPlants (Basel, Switzerland) · 2023Review
- New horizons for building pyrenoid-based CO2-concentrating mechanisms in plants to improve yields.Plant physiology · 2022Review
- Identification and Characterization of ATP-Binding Cassette Transporters inMarine drugs · 2022Article
4 more citing papers are in PubMed but not listed here.
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5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The supply of inorganic carbon (Ci; CO2 and HCO3 (-)) is an environmental rate-limiting factor in aquatic photosynthetic organisms. To overcome the difficulty in acquiring Ci in limiting-CO2 conditions, an active Ci uptake system called the CO2-concentrating mechanism (CCM) is induced to increase CO2 concentrations in the chloroplast stroma. An ATP-binding cassette transporter, HLA3, and a formate/nitrite transporter homolog, LCIA, are reported to be associated with HCO3 (-) uptake [Wang and Spalding (2014) Plant Physiol 166(4):2040-2050]. However, direct evidence of the route of HCO3 (-) uptake from the outside of cells to the chloroplast stroma remains elusive owing to a lack of information on HLA3 localization and comparative analyses of the contribution of HLA3 and LCIA to the CCM. In this study, we revealed that HLA3 and LCIA are localized to the plasma membrane and chloroplast envelope, respectively. Insertion mutants of HLA3 and/or LCIA showed decreased Ci affinities/accumulation, especially in alkaline conditions where HCO3 (-) is the predominant form of Ci. HLA3 and LCIA formed protein complexes independently, and the absence of LCIA decreased HLA3 mRNA accumulation, suggesting the presence of unidentified retrograde signals from the chloroplast to the nucleus to maintain HLA3 mRNA expression. Furthermore, although single overexpression of HLA3 or LCIA in high CO2 conditions did not affect Ci affinity, simultaneous overexpression of HLA3 with LCIA significantly increased Ci affinity/accumulation. These results highlight the HLA3/LCIA-driven cooperative uptake of HCO3 (-) and a key role of LCIA in the maintenance of HLA3 stability as well as Ci affinity/accumulation in the CCM.
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