ArticleInfection and immunity2015
Micropatterned macrophage analysis reveals global cytoskeleton constraints induced by Bacillus anthracis edema toxin.
Article in Infection and immunity, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 17 citations in OpenAlex.
- Anthrax ET activates Rac1 and RTK signaling to induce F-actin reorganization and endothelial permeability.iScience · 2025Article
- Review
- Quantifying spatial dynamics of Mycobacterium tuberculosis infection of human macrophages using microfabricated patterns.Cell reports methods · 2023Article
- Calcium Signaling in Pancreatic Immune CellsFunction (Oxford, England) · 2021Article
- Environmental Cues Modulate Microglial Cell Behavior Upon Shiga Toxin 2 From EnterohemorrhagicFrontiers in cellular and infection microbiology · 2019Article
- Contribution of the French army health service in support of expertise and research in infectiology in Africa.New microbes and new infections · 2018Article
- Nuclear morphologies: their diversity and functional relevance.Chromosoma · 2017Review
- Capturing extracellular matrix properties in vitro: Microengineering materials to decipher cell and tissue level processes.Experimental biology and medicine (Maywood, N.J.) · 2016Review
- The Saccharomyces boulardii CNCM I-745 strain shows protective effects against the B. anthracis LT toxin.Toxins · 2015Article
- Crossing of the epithelial barriers by Bacillus anthracis: the Known and the Unknown.Frontiers in microbiology · 2015Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacillus anthracis secretes the edema toxin (ET) that disrupts the cellular physiology of endothelial and immune cells, ultimately affecting the adherens junction integrity of blood vessels that in turn leads to edema. The effects of ET on the cytoskeleton, which is critical in cell physiology, have not been described thus far on macrophages. In this study, we have developed different adhesive micropatterned surfaces (L and crossbow) to control the shape of bone marrow-derived macrophages (BMDMs) and primary peritoneal macrophages. We found that macrophage F-actin cytoskeleton adopts a specific polar organization slightly different from classical human HeLa cells on the micropatterns. Moreover, ET induced a major quantitative reorganization of F-actin within 16 h with a collapse at the nonadhesive side of BMDMs along the nucleus. There was an increase in size and deformation into a kidney-like shape, followed by a decrease in size that correlates with a global cellular collapse. The collapse of F-actin was correlated with a release of focal adhesion on the patterns and decreased cell size. Finally, the cell nucleus was affected by actin reorganization. By using this technology, we could describe many previously unknown macrophage cellular dysfunctions induced by ET. This novel tool could be used to analyze more broadly the effects of toxins and other virulence factors that target the cytoskeleton.
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