ArticleAntimicrobial agents and chemotherapy2015
Effects of a Mutation in the gyrA Gene on the Virulence of Uropathogenic Escherichia coli.
Article in Antimicrobial agents and chemotherapy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 20 citations in OpenAlex.
- Antibiotic susceptibility and resistance genes in Escherichia coli from broilers reared in a low-antibiotic-use production system.Poultry science · 2026Article
- Antibiotic-induced gut microbiome dysbiosis: risks and strategies for mitigation.The EMBO journal · 2026Review
- Development and Validation of a High-Resolution Melting (HRM) Method for Differentiating Ovis and Equi Biovars ofVeterinary sciences · 2026Article
- The diverse phenotypic and mutational landscape induced by fluoroquinolone treatment.mSystems · 2025Article
- Distribution of Fimbrial Genes and Their Association with Virulence and Levofloxacin Resistance/Extended-Spectrum Beta-Lactamase Production in UropathogenicAntibiotics (Basel, Switzerland) · 2025Article
- A computational study exploring echinoderm-derived compounds for inhibition of aminoglycoside acetyltransferases.PloS one · 2025Article
- DNA supercoiling and regulation of intrinsic β-lactamase in pathogenic Escherichia coli.Archives of microbiology · 2023Review
- Recurrent bacteremia with a hypermucoviscous Escherichia coli isolated from a patient with perihilar cholangiocarcinoma: insights from a comprehensive genome-based analysis.Annals of clinical microbiology and antimicrobials · 2022Article
- Ciprofloxacin Pharmacokinetics/Pharmacodynamics against Susceptible and Low-Level Resistant Escherichia coli Isolates in an Experimental Ascending Urinary Tract Infection Model in Mice.Antimicrobial agents and chemotherapy · 2020Article
- Relationship between Virulence and Resistance among Gram-Negative Bacteria.Antibiotics (Basel, Switzerland) · 2020Review
- Molecular and Phenotypic Characterization ofAntibiotics (Basel, Switzerland) · 2020Article
- Ciprofloxacin, amoxicillin, and aminoglycosides stimulate genetic and phenotypic changes in uropathogenic Escherichia coli strains.Virulence · 2019Article
- The genetic background of antibiotic resistance among clinical uropathogenic Escherichia coli strains.Molecular biology reports · 2018Article
- DNA supercoiling is a fundamental regulatory principle in the control of bacterial gene expression.Biophysical reviews · 2016Review
- Article
- DNA supercoiling is a fundamental regulatory principle in the control of bacterial gene expression.Biophysical reviews · 2016Review
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluoroquinolones are among the drugs most extensively used for the treatment of bacterial infections in human and veterinary medicine. Resistance to quinolones can be chromosome or plasmid mediated. The chromosomal mechanism of resistance is associated with mutations in the DNA gyrase- and topoisomerase IV-encoding genes and mutations in regulatory genes affecting different efflux systems, among others. We studied the role of the acquisition of a mutation in the gyrA gene in the virulence and protein expression of uropathogenic Escherichia coli (UPEC). The HC14366M strain carrying a mutation in the gyrA gene (S83L) was found to lose the capacity to cause cystitis and pyelonephritis mainly due to a decrease in the expression of the fimA, papA, papB, and ompA genes. The levels of expression of the fimA, papB, and ompA genes were recovered on complementing the strain with a plasmid containing the gyrA wild-type gene. However, only a slight recovery was observed in the colonization of the bladder in the GyrA complement strain compared to the mutant strain in a murine model of ascending urinary tract infection. In conclusion, a mutation in the gyrA gene of uropathogenic E. coli reduced the virulence of the bacteria, likely in association with the effect of DNA supercoiling on the expression of several virulence factors and proteins, thereby decreasing their capacity to cause cystitis and pyelonephritis.
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