ArticleACS nano2015
Atomic Force Microscopy Mechanical Mapping of Micropatterned Cells Shows Adhesion Geometry-Dependent Mechanical Response on Local and Global Scales.
Article in ACS nano, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed.
- Measuring the Elastic Properties of Living Cells.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Lectin-Micropatterned Biointerfaces Regulate Cytoskeletal Organization and Viscoelastic Phenotypes of Bladder Cancer Cells.ACS omega · 2026Article
- Multiscale Architecture and Mechanics of the Cell Nucleus: Implications for Disease, Bioengineering and Nanomedicine.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Effect of two-dimensional spatial confinement on platelet mechanics.Biophysical reports · 2026Article
- CellMAP: an open-source software tool to batch-process cell topography and stiffness maps collected with an atomic force microscope.BMC bioinformatics · 2025Article
- Human septins organize as octamer-based filaments and mediate actin-membrane anchoring in cells.The Journal of cell biology · 2023Article
- ARHGEF9 regulates melanoma morphogenesis in environments with diverse geometry and elasticity by promoting filopodial-driven adhesion.iScience · 2022Article
- Submolecular probing of the complement C5a receptor-ligand binding reveals a cooperative two-site binding mechanism.Communications biology · 2020Article
- Cellular nanoscale stiffness patterns governed by intracellular forces.Nature materials · 2019Article
- Analyzing valve interstitial cell mechanics and geometry with spatial statistics.Journal of biomechanics · 2019Article
- Finite element simulation for the effect of loading rate on visco-hyperelastic characterisation of soft materials by spherical nanoindentation.IET nanobiotechnology · 2019Article
- Stiffness of MDCK II Cells Depends on Confluency and Cell Size.Biophysical journal · 2019Article
- Microfluidic Analysis for Separating and Measuring the Deformability of Cancer Cell Subpopulations.ACS omega · 2019Article
- Automated multi-sample acquisition and analysis using atomic force microscopy for biomedical applications.PloS one · 2019Article
- Determination of the Elastic Moduli of a Single Cell Cultured on a Rigid Support by Force Microscopy.Biophysical journal · 2018Article
- Mechanosensitivity of Cancer Cells in Contact with Soft Substrates Using AFM.Biophysical journal · 2018Article
- Modeling Ramp-hold Indentation Measurements based on Kelvin-Voigt Fractional Derivative Model.Measurement science & technology · 2018Article
- Single-Cell Defects Cause a Long-Range Mechanical Response in a Confluent Epithelial Cell Layer.Biophysical journal · 2017Article
- Broad modulus range nanomechanical mapping by magnetic-drive soft probes.Nature communications · 2017Article
- High-frequency microrheology reveals cytoskeleton dynamics in living cells.Nature physics · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
In multicellular organisms, cell shape and organization are dictated by cell-cell or cell-extracellular matrix adhesion interactions. Adhesion complexes crosstalk with the cytoskeleton enabling cells to sense their mechanical environment. Unfortunately, most of cell biology studies, and cell mechanics studies in particular, are conducted on cultured cells adhering to a hard, homogeneous, and unconstrained substrate with nonspecific adhesion sites, thus far from physiological and reproducible conditions. Here, we grew cells on three different fibronectin patterns with identical overall dimensions but different geometries (▽, T, and Y), and investigated their topography and mechanics by atomic force microscopy (AFM). The obtained mechanical maps were reproducible for cells grown on patterns of the same geometry, revealing pattern-specific subcellular differences. We found that local Young's moduli variations are related to the cell adhesion geometry. Additionally, we detected local changes of cell mechanical properties induced by cytoskeletal drugs. We thus provide a method to quantitatively and systematically investigate cell mechanics and their variations, and present further evidence for a tight relation between cell adhesion and mechanics.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.