ArticleMedical science monitor : international medical journal of experimental and clinical research2015
MiR-133 is Involved in Estrogen Deficiency-Induced Osteoporosis through Modulating Osteogenic Differentiation of Mesenchymal Stem Cells.
Article in Medical science monitor : international medical journal of experimental and clinical research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 52 citations in OpenAlex.
- MiRNAs regulate cell communication in osteogenesis-angiogenesis coupling during bone regeneration.Molecular biology reports · 2023Review
- Common miRNAs of Osteoporosis and Fibromyalgia: A Review.International journal of molecular sciences · 2023Review
- Gender-Specific Differences in Human Vertebral Bone Marrow Clot.International journal of molecular sciences · 2023Article
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- miR-4739/ITGA10/PI3K signaling regulates differentiation and apoptosis of osteoblast.Regenerative therapy · 2022Article
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- Small-extracellular vesicles and their microRNA cargo from porcine follicular fluids: the potential association with oocyte quality.Journal of animal science and biotechnology · 2022Article
- Influence of age on stem cells depends on the sex of the bone marrow donor.Journal of cellular and molecular medicine · 2022Article
- The Emerging Role of Non-Coding RNAs in Osteogenic Differentiation of Human Bone Marrow Mesenchymal Stem Cells.Frontiers in cell and developmental biology · 2022Review
- The Regulation of Collagen Processing by miRNAs in Disease and Possible Implications for Bone Turnover.International journal of molecular sciences · 2021Review
- Comparison Study of Stem Cell-Derived Extracellular Vesicles for Enhanced Osteogenic Differentiation.Tissue engineering. Part A · 2021Article
- MYOD1 inhibits avian adipocyte differentiation via miRNA-206/KLF4 axis.Journal of animal science and biotechnology · 2021Article
- Integrated analysis of miRNA and mRNA transcriptomic reveals antler growth regulatory network.Molecular genetics and genomics : MGG · 2021Article
- 17 β-Estradiol alleviates oxidative damage in osteoblasts by regulating miR-320/RUNX2 signaling pathway.Journal of biosciences · 2021Article
- SDF-1Stem cells international · 2021Article
- Plasma extracellular vesicle miRNAs as potential biomarkers of superstimulatory response in cattle.Scientific reports · 2020Article
- The Role of MicroRNAs in Bone Metabolism and Disease.International journal of molecular sciences · 2020Review
- Inhibition of microRNA-221-5p induces osteogenic differentiation by directly targeting smad3 in myeloma bone disease mesenchymal stem cells.Oncology letters · 2019Article
- Deregulated miRNAs in osteoporosis: effects in bone metastasis.Cellular and molecular life sciences : CMLS · 2019Review
- Effects of different intensities of strength and endurance training on some osteometabolic miRNAs, Runx2 and PPARγ in bone marrow of old male wistar rats.Molecular biology reports · 2019Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMiR-133 expression is dysregulated in postmenopausal osteoporosis. However, its role in postmenopausal osteoporosis is still not well understood. In the current study, we explore how estrogen deficiency affects miR-133 expression and how miR-133 is involved in osteogenic differentiation of mesenchymal stem cells (MSCs). MATERIAL AND
methodsqRT-PCR analysis was performed to assess miR-133 expression in MSCs isolated from bone marrow of an ovariectomized (OVX) animal model and postmenopausal osteoporosis patients (PMOP) and their corresponding controls. The binding between miR-133 and predicted target SLC39A1 was verified using dual luciferase assay and Western blot analysis. The effect of miR-133 and SLC39A1 on osteogenic differentiation of MSCs was assessed through measuring alkaline phosphatase (ALP), mineralization nodules, and osteoblast-specific genes Runx2 and Osterix expression.
resultsmiR-133 expression is significantly enhanced as a result of estrogen deficiency. Its overexpression is negatively correlated to osteogenic differentiation of hMSCs. SLC39A1 showed an inverse expression trend to miR-133 during the differentiation. miR-133 can directly target 3'UTR of SLC39A1 and thereby modulate its expression in hMSCs. The miR-133-SLC39A1 axis might play an important role in osteogenic differentiation of hMSCs. SLC39A1 can promote ALP activity and formation of mineralization nodules. In addition, SLC39A1 expression level is also positively correlated with RUNX2 and Osterix.
conclusionsEstrogen deficiency is associated with miR-133 overexpression. MiR-133 can induce postmenopausal osteoporosis by weakening osteogenic differentiation of hMSCs, at least partly through repressing SLC39A1 expression.
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