ArticleBMC biotechnology2015
Development of genetic tools for Myceliophthora thermophila.
Article in BMC biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Article
- High-throughput screening reveals mechanisms of environmental control of germination in a fungal thermophile.bioRxiv : the preprint server for biology · 2026Article
- Characterizing the Role ofMicroorganisms · 2024Article
- The transcriptional factor Clr-5 is involved in cellulose degradation through regulation of amino acid metabolism in Neurospora crassa.BMC biotechnology · 2023Article
- Development of an efficient protein expression system in the thermophilic fungus Myceliophthora thermophila.Microbial cell factories · 2023Article
- The putative methyltransferase LaeA regulates mycelium growth and cellulase production in Myceliophthora thermophila.Biotechnology for biofuels and bioproducts · 2023Article
- The arabinose transporter MtLat-1 is involved in hemicellulase repression as a pentose transceptor in Myceliophthora thermophila.Biotechnology for biofuels and bioproducts · 2023Article
- MtTRC-1, a Novel Transcription Factor, Regulates Cellulase Production via Directly Modulating the Genes Expression of theApplied and environmental microbiology · 2022Article
- Article
- Development of an Efficient C-to-T Base-Editing System and Its Application to Cellulase Transcription Factor Precise Engineering in Thermophilic FungusMicrobiology spectrum · 2022Article
- Establishment of High-Efficiency Screening System for Gene Deletion inJournal of fungi (Basel, Switzerland) · 2022Article
- Engineered FungusJournal of fungi (Basel, Switzerland) · 2022Article
- PFK2/FBPase-2 is a potential target for metabolic engineering in the filamentous fungusFrontiers in microbiology · 2022Article
- Coordination of consolidated bioprocessing technology and carbon dioxide fixation to produce malic acid directly from plant biomass in Myceliophthora thermophila.Biotechnology for biofuels · 2021Article
- Transcriptional Profiling ofFrontiers in microbiology · 2021Article
- Metabolic engineering of the cellulolytic thermophilic fungusBiotechnology for biofuels · 2020Article
- Development of genetic tools for the thermophilic filamentous fungusBiotechnology for biofuels · 2020Article
- STK-12 acts as a transcriptional brake to control the expression of cellulase-encoding genes in Neurospora crassa.PLoS genetics · 2019Article
- Dissecting cellobiose metabolic pathway and its application in biorefinery through consolidated bioprocessing inFungal biology and biotechnology · 2019Article
- Article
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7 authors.
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Abstract
backgroundThe thermophilic filamentous fungus Myceliophthora thermophila has many suitable characteristics for industrial biotechnology and could be a promising new chassis system for synthetic biology, particularly the ATCC 42464 strain, whose genome was sequenced in 2011. However, metabolic engineering of this strain using genetic approaches has not been reported owing to a lack of genetic tools for this organism.
resultsIn the present study, we developed a high efficiency Agrobacterium tumefaciens mediated transformation system for M. thermophila, including an approach for targeted gene deletion using green fluorescence protein (GFP) as a marker for selection. Up to 145 transformants per 10(5) conidia were obtained in one transformation plate. Moreover, a ku70 deletion mutant was constructed in the ATCC 42464 background using the tools developed in present study and subsequently characterized. The ku70 deletion construct was designed using resistance to phosphinothricin as the selection marker. Additionally, a GFP-encoding cassette was incorporated that allowed for the selection of site-specific (no fluorescence) or ectopic (fluorescence) integration of the ku70 construct. Transformants with ectopically integrated ku70 deletion constructs were therefore identified using the fluorescent signal of GFP. PCR and Southern blotting analyses of non-fluorescent putative ku70 deletion transformants revealed all 11 tested transformants to be correct deletions. The deletion frequency in a pool of 116 transformants analyzed was 58 %. Moreover, the homologous rate improved about 3 folds under ku70 mutant using the pyrG as a test gene to disrupt in M. thermophila.
conclusionsWe successfully developed an efficient transformation and target gene disruption approach for M. thermophila ATCC 42464 mediated by A. tumefaciens. The tools and the ku70 deletion strain developed here should advance the development of M. thermophila as an industrial host through metabolic engineering and accelerate the elucidation of the mechanism of rapid cellulose degradation in this thermophilic fungus.
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