ArticleScientific reports2015
A transcription and translation-coupled DNA replication system using rolling-circle replication.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 44 citations in OpenAlex.
- A self-replicating artificial module-genome that generates bacterial chromosome replication system in vitro.Nucleic acids research · 2026Article
- Reconstituting transcription-translation-coupled DNA replication within complex in vitro biological systems.Nature communications · 2025Article
- Genetically Encoded Control ofACS synthetic biology · 2025Article
- Simultaneous in vitro expression of minimal 21 transfer RNAs by tRNA array method.Nature communications · 2025Article
- C2CAplus: A One-Pot Isothermal Circle-to-Circle DNA Amplification System.ACS synthetic biology · 2023Article
- Clonal Amplification-Enhanced Gene Expression in Synthetic Vesicles.ACS synthetic biology · 2023Article
- The Long Road to a Synthetic Self-Replicating Central Dogma.Biochemistry · 2023Review
- Article
- Build-a-Cell: Engineering a Synthetic Cell Community.Life (Basel, Switzerland) · 2021Article
- Towards a synthetic cell cycle.Nature communications · 2021Review
- In vitro self-replication and multicistronic expression of large synthetic genomes.Nature communications · 2020Article
- Cell-Free Systems: A Proving Ground for Rational Biodesign.Frontiers in bioengineering and biotechnology · 2020Article
- Bottom-Up Construction of Complex Biomolecular Systems With Cell-Free Synthetic Biology.Frontiers in bioengineering and biotechnology · 2020Review
- Self-replication of circular DNA by a self-encoded DNA polymerase through rolling-circle replication and recombination.Scientific reports · 2018Article
- Automated in vitro evolution of a translation-coupled RNA replication system in a droplet flow reactor.Scientific reports · 2018Article
- Self-replication of DNA by its encoded proteins in liposome-based synthetic cells.Nature communications · 2018Article
- Are in vivo selections on the path to extinction?Microbial biotechnology · 2017Article
- Synthetic biology approaches to biological containment: pre-emptively tackling potential risks.Essays in biochemistry · 2016Review
- Constructive Approaches for Understanding the Origin of Self-Replication and Evolution.Life (Basel, Switzerland) · 2016Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
All living organisms have a genome replication system in which genomic DNA is replicated by a DNA polymerase translated from mRNA transcribed from the genome. The artificial reconstitution of this genome replication system is a great challenge in in vitro synthetic biology. In this study, we attempted to construct a transcription- and translation-coupled DNA replication (TTcDR) system using circular genomic DNA encoding phi29 DNA polymerase and a reconstituted transcription and translation system. In this system, phi29 DNA polymerase was translated from the genome and replicated the genome in a rolling-circle manner. When using a traditional translation system composition, almost no DNA replication was observed, because the tRNA and nucleoside triphosphates included in the translation system significantly inhibited DNA replication. To minimize these inhibitory effects, we optimized the composition of the TTcDR system and improved replication by approximately 100-fold. Using our system, genomic DNA was replicated up to 10 times in 12 hours at 30 °C. This system provides a step toward the in vitro construction of an artificial genome replication system, which is a prerequisite for the construction of an artificial cell.
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