Evidence map›Paper›PMID 26012633›Full record

ArticleScience signaling2015

A large-scale screen reveals genes that mediate electrotaxis in Dictyostelium discoideum.

Runchi Gao, Siwei Zhao, Xupin Jiang, Yaohui Sun, Sanjun Zhao, Jing Gao, Jane Borleis, Stacey Willard, Ming Tang, Huaqing Cai and 8 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Science signaling, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 49 citations in OpenAlex.

  1. Article
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  6. Article
  7. Article
  8. Review
  9. Article
  10. Review
  11. Article
  12. Article
  13. Review
  14. The principles of directed cell migration.Nature reviews. Molecular cell biology · 2021
    Review
  15. The Dynamics of Aerotaxis in a Simple Eukaryotic Model.Frontiers in cell and developmental biology · 2021
    Article
  16. Biomedical applications of electrical stimulation.Cellular and molecular life sciences : CMLS · 2020
    Review
  17. Article
  18. Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors at 4 institutions in 2 countries.

Runchi GaoSchool of Life Sciences, Yunnan Normal University, Kunming, Yunnan 650500, China. Departments of Dermatology and Ophthalmology, Institute for Regenerative Cures, School of Medicine, University of California at Davis, Davis, CA 95817, USA. Department of Cell Biology and Anatomy, Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.
Siwei ZhaoDepartment of Biomedical Engineering, University of California at Davis, Davis, CA 95616, USA.
Xupin JiangState Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University, Chongqing 400042, China.
Yaohui SunDepartments of Dermatology and Ophthalmology, Institute for Regenerative Cures, School of Medicine, University of California at Davis, Davis, CA 95817, USA.
Sanjun ZhaoSchool of Life Sciences, Yunnan Normal University, Kunming, Yunnan 650500, China. Departments of Dermatology and Ophthalmology, Institute for Regenerative Cures, School of Medicine, University of California at Davis, Davis, CA 95817, USA.
Jing GaoSchool of Life Sciences, Yunnan Normal University, Kunming, Yunnan 650500, China. Departments of Dermatology and Ophthalmology, Institute for Regenerative Cures, School of Medicine, University of California at Davis, Davis, CA 95817, USA.
Jane BorleisDepartment of Cell Biology and Anatomy, Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.
Stacey WillardDepartment of Cell Biology and Anatomy, Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.
Ming TangDepartment of Cell Biology and Anatomy, Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.
Huaqing CaiDepartment of Cell Biology and Anatomy, Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.
Yoichiro KamimuraDepartment of Cell Biology and Anatomy, Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.
Yuesheng HuangState Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University, Chongqing 400042, China.
Jianxin JiangState Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University, Chongqing 400042, China.
Zunxi HuangSchool of Life Sciences, Yunnan Normal University, Kunming, Yunnan 650500, China.
Alex MogilnerCourant Institute and Department of Biology, New York University, 251 Mercer Street, New York, NY 10012, USA.
Tingrui PanDepartment of Biomedical Engineering, University of California at Davis, Davis, CA 95616, USA.
Peter N DevreotesDepartment of Cell Biology and Anatomy, Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.
Min ZhaoDepartments of Dermatology and Ophthalmology, Institute for Regenerative Cures, School of Medicine, University of California at Davis, Davis, CA 95817, USA. minzhao@ucdavis.edu.
Yunnan Normal University · CNJohns Hopkins University · USArmy Medical University · CNCalifornia Institute for Regenerative Medicine · US

Funding

STRUCTUREU54GM064346 · NIGMS · UNIVERSITY OF VIRGINIA CHARLOTTESVILLE · PI IMPERIALI, BARBARA · 2001 to 2010
$74.9M
Excitable Networks in Directed Cell MigrationR35GM118177 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI Peter N Devreotes · 2016 to 2026
$12.1M
Temporal and Spatial Signaling in ChemotaxisR01GM034933 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI DEVREOTES, PETER N · 1987 to 2015
$8.8M
Molecular Generators at Corneal Wounds Produce and regulate the Wound Electrical SignalsR01EY019101 · NEI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI ZHAO, MIN · 2010 to 2023
$5.2M
Modeling mitotic spindle assemblyR01GM068952 · NIGMS · UNIVERSITY OF CALIFORNIA DAVIS · PI MOGILNER, ALEXANDER · 2003 to 2014
$4.4M
SIGNAL TRANSDUCTION AND DICTYOSTELIUM DEVELOPMENTR01GM028007 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI DEVREOTES, PETER N · 1985 to 2004
$2.4M
Guiding angiogenesisR21EB015737 · NIBIB · UNIVERSITY OF CALIFORNIA AT DAVIS · PI ZHAO, MIN · 2013 to 2014
$418k
NEI NIH HHS 1R01EY019101NEI NIH HHS R01 EY019101NIBIB NIH HHS EB015737NIGMS NIH HHS GM64346NIGMS NIH HHS R01 GM028007NIGMS NIH HHS R01 GM034933NIGMS NIH HHS R01 GM068952NIGMS NIH HHS R35 GM118177NIGMS NIH HHS U54 GM064346Wellcome TrustWellcome Trust WT082887MA
6 · The paper itself

Abstract

Directional cell migration in an electric field, a phenomenon called galvanotaxis or electrotaxis, occurs in many types of cells, and may play an important role in wound healing and development. Small extracellular electric fields can guide the migration of amoeboid cells, and we established a large-scale screening approach to search for mutants with electrotaxis phenotypes from a collection of 563 Dictyostelium discoideum strains with morphological defects. We identified 28 strains that were defective in electrotaxis and 10 strains with a slightly higher directional response. Using plasmid rescue followed by gene disruption, we identified some of the mutated genes, including some previously implicated in chemotaxis. Among these, we studied PiaA, which encodes a critical component of TORC2, a kinase protein complex that transduces changes in motility by activating the kinase PKB (also known as Akt). Furthermore, we found that electrotaxis was decreased in mutants lacking gefA, rasC, rip3, lst8, or pkbR1, genes that encode other components of the TORC2-PKB pathway. Thus, we have developed a high-throughput screening technique that will be a useful tool to elucidate the molecular mechanisms of electrotaxis.

Indexed as

DictyosteliumMultiprotein ComplexesProto-Oncogene Proteins c-aktProtozoan ProteinsTOR Serine-Threonine KinasesGene Knockdown TechniquesMechanistic Target of Rapamycin Complex 2MutationSignal TransductionMechanistic Target of Rapamycin Complex 2Multiprotein ComplexesProto-Oncogene Proteins c-aktProtozoan ProteinsTOR Serine-Threonine Kinases

Identifiers

PMID26012633
PMCPMC4470479
OpenAlexW2236576493

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.