ArticlePloS one2015
Complete Genome Sequence of ER2796, a DNA Methyltransferase-Deficient Strain of Escherichia coli K-12.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- DNA modifications of Durham Collection phages and promiscuity of GmrSD-family Type IV restriction enzyme BrxU.Applied and environmental microbiology · 2026Article
- Multiple forms of protein-protein and DNA binding are exhibited by BrxC from the BREX phage restriction system.Nucleic acids research · 2026Article
- Multi-gene Co-expression systems inSynthetic and systems biotechnology · 2026Review
- Competing forms of protein-protein association and DNA binding exhibited by BrxC from the BREX phage restriction system.bioRxiv : the preprint server for biology · 2026Article
- A pentose, as a cytosine nucleobase modification inApplied and environmental microbiology · 2026Article
- Enhanced Transformation Efficiency in Fusobacterium nucleatum by Bypassing Restriction-Modification Systems: A Methylation-Guided Protocol.Methods in molecular biology (Clifton, N.J.) · 2026Article
- PglZ from Type I BREX phage defence systems is a metal-dependent nuclease that forms a sub-complex with BrxB.Nucleic acids research · 2025Article
- Evolution of a Plasmid Regulatory Circuit Ameliorates Plasmid Fitness Cost.Molecular biology and evolution · 2025Article
- DNA-m6A calling and integrated long-read epigenetic and genetic analysis withGenome research · 2024Article
- Critical assessment of nanopore sequencing for the detection of multiple forms of DNA modifications.bioRxiv : the preprint server for biology · 2024Article
- Structure and rational engineering of the PglX methyltransferase and specificity factor for BREX phage defence.Nature communications · 2024Article
- Evolution of a Plasmid Regulatory Circuit Ameliorates Plasmid Fitness Cost.bioRxiv : the preprint server for biology · 2024Article
- Enhanced Fusobacterium nucleatum Genetics Using Host DNA Methyltransferases To Bypass Restriction-Modification Systems.Journal of bacteriology · 2022Article
- Tuning of Gene Expression inACS synthetic biology · 2022Article
- Critical assessment of DNA adenine methylation in eukaryotes using quantitative deconvolution.Science (New York, N.Y.) · 2022Article
- Article
- Genome archaeology of two laboratory Salmonella enterica enterica sv Typhimurium.G3 (Bethesda, Md.) · 2021Article
- Article
- Two DNA Methyltransferases for Site-Specific 6mA and 5mC DNA Modification inFrontiers in plant science · 2021Article
- Plasmid replication-associated single-strand-specific methyltransferases.Nucleic acids research · 2020Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report the complete sequence of ER2796, a laboratory strain of Escherichia coli K-12 that is completely defective in DNA methylation. Because of its lack of any native methylation, it is extremely useful as a host into which heterologous DNA methyltransferase genes can be cloned and the recognition sequences of their products deduced by Pacific Biosciences Single-Molecule Real Time (SMRT) sequencing. The genome was itself sequenced from a long-insert library using the SMRT platform, resulting in a single closed contig devoid of methylated bases. Comparison with K-12 MG1655, the first E. coli K-12 strain to be sequenced, shows an essentially co-linear relationship with no major rearrangements despite many generations of laboratory manipulation. The comparison revealed a total of 41 insertions and deletions, and 228 single base pair substitutions. In addition, the long-read approach facilitated the surprising discovery of four gene conversion events, three involving rRNA operons and one between two cryptic prophages. Such events thus contribute both to genomic homogenization and to bacteriophage diversification. As one of relatively few laboratory strains of E. coli to be sequenced, the genome also reveals the sequence changes underlying a number of classical mutant alleles including those affecting the various native DNA methylation systems.
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