ArticleProteomics2015
Pitfalls in histone propionylation during bottom-up mass spectrometry analysis.
Article in Proteomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Rapid Histone Post-Translational Modification Analysis Using Alternative Proteases and Tandem Mass Tags.Analytical chemistry · 2026Article
- Rapid Histone Post-Translational Modification Analysis Using Alternative Proteases and Tandem Mass Tags.bioRxiv : the preprint server for biology · 2026Article
- Lysine Propionylation as a Metabolically Coupled PTM: Mechanisms, Functional Consequences, and Therapeutic Potentials.International journal of molecular sciences · 2026Review
- Breaking Boundaries in Histone Modification MS-Based Detection: A Tailored Search Strategy for Unrestricted Identification of Novel Epigenetic Marks.Molecular & cellular proteomics : MCP · 2025Article
- Normalized and Directional Interplay Scoring for the Interrogation of Proteoform Data.Journal of proteome research · 2025Article
- An Optimized and High-Throughput Method for Histone Propionylation and Data-Independent Acquisition Analysis for the Identification and Quantification of Histone Post-translational Modifications.Journal of the American Society for Mass Spectrometry · 2023Article
- A Rapid and Efficient Method for the Extraction of Histone Proteins.Journal of proteome research · 2023Article
- Mass Spectrometry Contribution to Pediatric Cancers Research.Medicina (Kaunas, Lithuania) · 2023Review
- Micro-Topographies Induce Epigenetic Reprogramming and Quiescence in Human Mesenchymal Stem Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- An interactive mass spectrometry atlas of histone posttranslational modifications in T-cell acute leukemia.Scientific data · 2022Article
- A large scale mass spectrometry-based histone screening for assessing epigenetic developmental toxicity.Scientific reports · 2022Article
- Histone Sample Preparation for Bottom-Up Mass Spectrometry: A Roadmap to Informed Decisions.Proteomes · 2021Article
- Trimethylacetic Anhydride-Based Derivatization Facilitates Quantification of Histone Marks at the MS1 Level.Molecular & cellular proteomics : MCP · 2021Article
- Mass Spectrometry to Study Chromatin Compaction.Biology · 2020Review
- Comprehensive histone epigenetics: A mass spectrometry based screening assay to measure epigenetic toxicity.MethodsX · 2020Article
- Untargeted histone profiling during naive conversion uncovers conserved modification markers between mouse and human.Scientific reports · 2019Article
- Filter-Aided Sample Preparation Procedure for Mass Spectrometric Analysis of Plant Histones.Frontiers in plant science · 2018Article
- Recent Achievements in Characterizing the Histone Code and Approaches to Integrating Epigenomics and Systems Biology.Methods in enzymology · 2017Review
- Extracting histones for the specific purpose of label-free MS.Proteomics · 2016Article
- Epiproteomics: quantitative analysis of histone marks and codes by mass spectrometry.Current opinion in chemical biology · 2016Review
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite their important role in regulating gene expression, posttranslational histone modifications remain technically challenging to analyze. For identification by bottom-up MS, propionylation is required prior to and following trypsin digestion. Hereby, more hydrophobic peptides are generated enabling RP HPLC separation. When histone dynamics are studied in a quantitative manner, specificity, and efficiency of this chemical derivatization are crucial. Therefore we examined eight different protocols, including two different propionylation reagents. This revealed amidation (up to 70%) and methylation (up to 9%) of carboxyl groups as a side reaction. Moreover, incomplete (up to 85%) as well as a specific propionylation (up to 63%) can occur, depending on the protocol. These results highlight the possible pitfalls and implications for data analysis when doing bottom-up MS on histones.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.