ArticleObesity (Silver Spring, Md.)2015
Skeletal muscle myotubes in severe obesity exhibit altered ubiquitin-proteasome and autophagic/lysosomal proteolytic flux.
Article in Obesity (Silver Spring, Md.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 45 citations in OpenAlex.
- A randomized clinical trial on the effects of exercise on muscle remodelling following bariatric surgery.Journal of cachexia, sarcopenia and muscle · 2021Trial
- The Human Skeletal Muscle Transcriptome in Response to Oral Shilajit Supplementation.Journal of medicinal food · 2016Trial
- Bortezomib exhibited bacteriostatic activity and pro-inflammatory effect against Mycoplasma pneumoniae.The Journal of antibiotics · 2026Article
- Obesity accelerates aging: Mechanisms and therapeutic implications.Genes & diseases · 2026Review
- Emerging perspectives in proteostasis: bridging mechanisms and therapeutics for human diseases.Signal transduction and targeted therapy · 2026Review
- Muscle Insulin Resistance Elicits Muscle Atrophy in Obesity.Current obesity reports · 2025Review
- Fiber-specific differences in protein content of pathways related to mTORC1 signaling and oxidative metabolism in individuals with obesity.Scientific reports · 2025Article
- Autolysosomal Dysfunction in Obesity-induced Metabolic Inflammation and Related Disorders.Current obesity reports · 2025Review
- The Effect of Maternal Obesity on Placental Autophagy in Lean Breed Sows.Veterinary sciences · 2025Article
- Uric acid formation is driven by crosstalk between skeletal muscle and other cell types.JCI insight · 2024Article
- Muscle fiber phenotype: a culprit of abnormal metabolism and function in skeletal muscle of humans with obesity.American journal of physiology. Endocrinology and metabolism · 2023Review
- Melatonin Improves Skeletal Muscle Structure and Oxidative Phenotype by Regulating Mitochondrial Dynamics and Autophagy in Zücker Diabetic Fatty Rat.Antioxidants (Basel, Switzerland) · 2023Article
- Prevalence and Mechanisms of Skeletal Muscle Atrophy in Metabolic Conditions.International journal of molecular sciences · 2023Review
- T-cell Metabolism as Interpreted in Obesity-associated Inflammation.Endocrinology · 2022Article
- Primary skeletal muscle cells from chronic kidney disease patients retain hallmarks of cachexia in vitro.Journal of cachexia, sarcopenia and muscle · 2022Article
- Histone deacetylase 6 inhibition restores leptin sensitivity and reduces obesity.Nature metabolism · 2022Article
- Magnesium Lithospermate B Attenuates High-Fat Diet-Induced Muscle Atrophy in C57BL/6J Mice.Nutrients · 2021Article
- MicroRNA-204-5p modulates mitochondrial biogenesis in C2C12 myotubes and associates with oxidative capacity in humans.Journal of cellular physiology · 2020Article
- Increased AMP deaminase activity decreases ATP content and slows protein degradation in cultured skeletal muscle.Metabolism: clinical and experimental · 2020Article
- Mitochondrial Function in Muscle Stem Cell Fates.Frontiers in cell and developmental biology · 2020Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
objectiveWhole-body protein metabolism is dysregulated with obesity. The goal of the study was to determine whether activity and expression of major protein degradation pathways are compromised specifically in human skeletal muscle with obesity.
methodsPrimary human skeletal muscle (HSkM) cell cultures were utilized since cellular mechanisms can be studied absent of hormones and contractile activity that could independently influence metabolism. HSkM from 10 lean women (BMI ≤ 26.0 kg/m(2) ) and 8 women with severe obesity (BMI ≥ 39.0) were examined basally and when stimulated to atrophy (serum and amino acid starvation).
resultsHSkM from obese donors had a lower proportion of type I myosin heavy chain and slower flux through the autophagic/lysosomal pathway. During starvation, flux through the ubiquitin-proteasome system diverged according to obesity status, with a decrease in lean subjects and an increase in HSkM from subjects with obesity. HSkM in obesity also displayed elevated proteasome activity despite no difference in proteasome content. Atrophy-related gene expression and myotube area were similar in myotubes derived from individuals with and without obesity under basal and starved conditions.
conclusionsOur data indicate that muscle cells in lean individuals and in those with severe obesity have innate differences in management of protein degradation, which may explain their metabolic differences.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.