Evidence map›Paper›PMID 26010154›Full record

ArticlePloS one2015

Up-Regulation of miR-21 Is Associated with Cervicitis and Human Papillomavirus Infection in Cervical Tissues.

Sureewan Bumrungthai, Tipaya Ekalaksananan, Mark Francis Evans, Peechanika Chopjitt, Thumwadee Tangsiriwatthana, Natcha Patarapadungkit, Pilaiwan Kleebkaow, Sanguanchoke Luanratanakorn, Bunkerd Kongyingyoes, Suchin Worawichawong and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed, 2 pooled it
2.5field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed, 2 syntheses or guidelines pooled it, 65 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 2 countries.

Sureewan BumrungthaiDepartment of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; HPV & EBV and carcinogenesis Research Group, Khon Kaen University, Khon Kaen, Thailand.
Tipaya EkalaksanananDepartment of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; HPV & EBV and carcinogenesis Research Group, Khon Kaen University, Khon Kaen, Thailand.
Mark Francis EvansDepartment of Pathology and Laboratory Medicine, University of Vermont College of Medicine, Burlington, Vermont, United States of America.
Peechanika ChopjittDepartment of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; HPV & EBV and carcinogenesis Research Group, Khon Kaen University, Khon Kaen, Thailand.
Thumwadee TangsiriwatthanaDepartment of Obstetrics and Gynecology, Khon Kaen Hospital, Khon Kaen, Thailand; HPV & EBV and carcinogenesis Research Group, Khon Kaen University, Khon Kaen, Thailand.
Natcha PatarapadungkitDepartment of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; HPV & EBV and carcinogenesis Research Group, Khon Kaen University, Khon Kaen, Thailand.
Pilaiwan KleebkaowDepartment of Obstetrics and Gynecology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; HPV & EBV and carcinogenesis Research Group, Khon Kaen University, Khon Kaen, Thailand.
Sanguanchoke LuanratanakornDepartment of Obstetrics and Gynecology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Bunkerd KongyingyoesDepartment of Pharmacology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Suchin WorawichawongDepartment of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
Chamsai PientongDepartment of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; HPV & EBV and carcinogenesis Research Group, Khon Kaen University, Khon Kaen, Thailand.
Khon Kaen University · THMahidol University · THUniversity of Vermont · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

MicroRNA-21 (miR-21) is recognized as an oncomir and shows up-regulation in many types of human malignancy. The aim of this study was to investigate the association of miR-21 expression associated with HPV infection in normal and abnormal cervical tissues. Cervical tissue samples with different cytological or histopathological grades were investigated for HPV by PCR and for miR-21 and programmed cell death, protein 4 (PDCD4) expression using quantitative real-time PCR (qRT-PCR). Laser capture microdissection (LCM) of stromal and epithelial tissues and in situ hybridization (ISH) using locked nucleic acid (LNA) probes were performed on a subset of fixed specimens. Cell line experiments were conducted on fibroblasts stimulated in culture media from HeLa cells, which were then assessed for miR-21, PDCD4, IL-6 and α-SMA expression by qRT-PCR. Twenty normal cervical cell, 12 cervicitis, 14 cervical intraepithelial neoplastic I (CIN I), 22 CIN II-III and 43 cervical squamous cell carcinoma (SCC) specimens were investigated. miR-21 levels were significantly lower in normal than in abnormal tissues. The expression of miR-21 in HPV negative normal cytology was significantly lower than in HPV positive samples in abnormal tissue and SCC. The miR-21 expression was significantly higher in HPV negative cervicitis than HPV negative normal cells. LCM and ISH data showed that miR-21 is primarily expressed in the tumor-associated stromal cell microenvironment. Fibroblasts treated with HeLa cell culture media showed up-regulated expression of miR-21, which correlated with increased expression of α-SMA and IL-6 and with down-regulation of PDCD4. These results demonstrate that miR-21 is associated with HPV infection and involved in cervical lesions as well as cervicitis and its up-regulation in tumor-stroma might be involved in the inflammation process and cervical cancer progression.

Indexed as

ActinsApoptosis Regulatory ProteinsCarcinoma, Squamous CellCell Line, TumorCervix UteriDown-RegulationFemaleFibroblastsHeLa CellsHumansInterleukin-6MicroRNAsPapillomaviridaePapillomavirus InfectionsRNA-Binding ProteinsUp-RegulationACTA2 protein, humanActinsApoptosis Regulatory ProteinsInterleukin-6MicroRNAsMIRN21 microRNA, humanPDCD4 protein, humanRNA-Binding Proteins

Identifiers

PMID26010154
PMCPMC4444121
OpenAlexW1520719781

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.