ArticlePloS one2015
A novel role for DNA methyltransferase 1 in regulating oocyte cytoplasmic maturation in pigs.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Impact of Maturation and Vitrification Time of Human GV Oocytes on the Metaphase Plate Configuration.International journal of molecular sciences · 2021Trial
- Dnmt3b and Dnmt3l knockdown reduces blastocyst development in early mouse embryos.Histochemistry and cell biology · 2026Article
- Pramel15 facilitates zygotic nuclear DNMT1 degradation and DNA demethylation.Nature communications · 2024Article
- Trehalose delivered by cold-responsive nanoparticles improves tolerance of cumulus-oocyte complexes to microwave drying.Journal of assisted reproduction and genetics · 2023Article
- Melatonin protects against defects induced by Enniatin B1 during porcine early embryo development.Aging · 2021Article
- Pterostilbene Alleviates Chlorpyrifos-Induced Damage During Porcine Oocyte Maturation.Frontiers in cell and developmental biology · 2021Article
- Human exhaled air can efficiently supportAnimal reproduction · 2018Article
- Heat shock protein 90α couples with the MAPK-signaling pathway to determine meiotic maturation of porcine oocytes.Journal of animal science · 2018Article
- Single-cell transcriptome sequencing reveals that cell division cycle 5-like protein is essential for porcine oocyte maturation.The Journal of biological chemistry · 2018Article
- Imprinting disorder in donor cells is detrimental to the development of cloned embryos in pigs.Oncotarget · 2017Article
- Dnmt1s in donor cells is a barrier to SCNT-mediated DNA methylation reprogramming in pigs.Oncotarget · 2017Article
- Ovulation Statuses of Surrogate Gilts Are Associated with the Efficiency of Excellent Pig Cloning.PloS one · 2015Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Maternal factors are required for oocyte maturation and embryo development. To better understand the role of DNA methyltransferase 1 (Dnmt1) in oocyte maturation and embryo development, small interfering RNA (siRNA) was conducted in porcine oocytes. In this study, our results showed that Dnmt1 localized in oocyte cytoplasm and its expression displayed no obvious change during oocyte maturation. When siRNAs targeting Dnmt1 were injected into germinal vesicle (GV) stage oocytes, Dnmt1 transcripts significantly decreased in matured oocytes (P<0.05). After Dnmt1 knockdown in GV stage oocytes, the significant reduction of glutathione content, mitochondrial DNA copy number, glucose-6-phosphate dehydrogenase activity and expression profiles of maternal factors and the severely disrupted distribution of cortical granules were observed in MII stage oocytes (P<0.05), leading to the impaired oocyte cytoplasm. Further study displayed that Dnmt1 knockdown in GV stage oocytes significantly reduced the development of early embryos generated through parthenogenetic activation, in vitro fertilization and somatic cell nuclear transfer (P<0.05). In conclusion, Dnmt1 was indispensable for oocyte cytoplasmic maturation, providing a novel role for Dnmt1 in the regulation of oocyte maturation.
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