ArticleShock (Augusta, Ga.)2015
Whole Blood Reveals More Metabolic Detail of the Human Metabolome than Serum as Measured by 1H-NMR Spectroscopy: Implications for Sepsis Metabolomics.
Article in Shock (Augusta, Ga.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
44 citing papers in PubMed, 69 citations in OpenAlex.
- Pre-treatment amino acids and risk of paclitaxel-induced peripheral neuropathy in SWOG S0221.Cancer chemotherapy and pharmacology · 2024Trial
- Nutritional Metabolomics: Postprandial Response of Meals Relating to Vegan, Lacto-Ovo Vegetarian, and Omnivore Diets.Nutrients · 2018Trial
- Cryoprotective strategies for frozen erythrocytes in POCT whole blood reference materials progress and challenges.Analytical and bioanalytical chemistry · 2026Review
- Impact of blood contamination on hydrophilic metabolomics in human meningioma tissue.Analytical and bioanalytical chemistry · 2026Article
- Optimization of a targeted metabolomics kit for dried blood spots analysis and longitudinal comparison with serum.Metabolomics : Official journal of the Metabolomic Society · 2026Article
- Pitfalls of onco-metabolomics: impact of sample integrity on metabolomic investigations in more than 4500 human serum samples from ten different cohorts.Frontiers in molecular biosciences · 2026Article
- Quantitative comparison of whole blood, plasma and serum metabolomes across different blood collection methods.Metabolomics : Official journal of the Metabolomic Society · 2025Article
- Dry blood spots as a sampling strategy to identify insulin resistance markers during a dietary challenge.Genes & nutrition · 2024Article
- An Overview of Pre-Analytical Factors Impacting Metabolomics Analyses of Blood Samples.Metabolites · 2024Review
- MetaboAnalystR 4.0: a unified LC-MS workflow for global metabolomics.Nature communications · 2024Article
- Serum Metabolomic Profiles in Critically Ill Patients with Shock on Admission to the Intensive Care Unit.Metabolites · 2023Article
- Towards inferring absolute concentrations from relative abundance in time-course GC-MS metabolomics data.Molecular omics · 2023Article
- Stability Studies of Kynurenine Pathway Metabolites in Blood Components Define Optimal Blood Processing Conditions.International journal of tryptophan research : IJTR · 2023Article
- Metabolomics as a promising tool for improving understanding of multiple sclerosis: A review of recent advances.Biomedical journal · 2022Review
- Identifying potential biomarkers and therapeutic targets for dogs with sepsis using metabolomics and lipidomics analyses.PloS one · 2022Observational
- Review
- Whole-blood metabolomics of dementia patients reveal classes of disease-linked metabolites.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Article
- Diffuse Optical Spectroscopy Assessment of Resting Oxygen Metabolism in the Leg Musculature.Metabolites · 2021Article
- Analytical considerations for reducing the matrix effect for the sphingolipidome quantification in whole blood.Bioanalysis · 2021Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Serum is a common sample of convenience for metabolomics studies. Its processing time can be lengthy and may result in the loss of metabolites including those of red blood cells (RBCs). Unlike serum, whole blood (WB) is quickly processed, minimizing the influence of variable hemolysis while including RBC metabolites. To determine differences between serum and WB metabolomes, both sample types, collected from healthy volunteers, were assayed by H-NMR (proton nuclear magnetic resonance) spectroscopy. A total of 34 and 50 aqueous metabolites were quantified from serum and WB, respectively. Free hemoglobin (Hgb) levels in serum were measured, and the correlation between Hgb and metabolite concentrations was determined. Most metabolites detected in serum were at higher concentrations in WB with the exception of acetoacetate and propylene glycol. The 18 unique metabolites of WB included adenosine, AMP, ADP, and ATP, which are associated with RBC metabolism. The use of serum results in the underrepresentation of a number of metabolic pathways including branched-chain amino acid degradation and glycolysis and gluconeogenesis. The range of free Hgb in serum was 0.03 to 0.01 g/dL, and eight metabolites were associated (P ≤ 0.05) with free Hgb. The range of free Hgb in serum samples from 18 sepsis patients was 0.02 to 0.46 g/dL. Whole blood and serum have unique aqueous metabolite profiles, but the use of serum may introduce potential pathway bias. Use of WB for metabolomics may be particularly important for studies in diseases such as sepsis in which RBC metabolism is altered, and mechanical and sepsis-induced hemolysis contributes to variance in the metabolome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.