ArticleThe Journal of cell biology2015
Nuclear accessibility of β-actin mRNA is measured by 3D single-molecule real-time tracking.
Article in The Journal of cell biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed.
- Tetrahedral serial multiview microscopy and image fusion for improved resolution and extent in stained zebrafish embryos.Developmental dynamics : an official publication of the American Association of Anatomists · 2024Article
- Recent Advances in Single-Molecule Tracking and Imaging Techniques.Annual review of analytical chemistry (Palo Alto, Calif.) · 2023Review
- Design and Study of a Reflector-Separated Light Dispersion-Compensated 3D Microscopy System.Sensors (Basel, Switzerland) · 2023Article
- Cap-dependent translation initiation monitored in living cells.Nature communications · 2022Article
- Review
- Fast and parallel nanoscale three-dimensional tracking of heterogeneous mammalian chromatin dynamics.Molecular biology of the cell · 2022Article
- RNA transport from transcription to localized translation: a single molecule perspective.RNA biology · 2021Review
- Selective dendritic localization of mRNA ineLife · 2021Article
- Light Sheet Illumination for 3D Single-Molecule Super-Resolution Imaging of Neuronal Synapses.Frontiers in synaptic neuroscience · 2021Review
- Multiplex live single-cell transcriptional analysis demarcates cellular functional heterogeneity.eLife · 2019Article
- BigStitcher: reconstructing high-resolution image datasets of cleared and expanded samples.Nature methods · 2019Article
- Recent progress in single-molecule studies of mRNA localizationRNA biology · 2019Review
- MS2 Labeling of Endogenous Beta-Actin mRNA Does Not Result in Stabilization of Degradation Intermediates.Molecules and cells · 2019Article
- An automated Bayesian pipeline for rapid analysis of single-molecule binding data.Nature communications · 2019Article
- Advanced 3D Analysis and Optimization of Single-Molecule FISH inSmall methods · 2018Article
- Accurate and fiducial-marker-free correction for three-dimensional chromatic shift in biological fluorescence microscopy.Scientific reports · 2018Article
- RNP transport in cell biology: the long and winding road.Current opinion in cell biology · 2017Review
- Temporal and spatial regulation of mRNA export: Single particle RNA-imaging provides new tools and insights.BioEssays : news and reviews in molecular, cellular and developmental biology · 2017Review
- Single-Cell and Single-Molecule Analysis of Gene Expression Regulation.Annual review of genetics · 2016Review
- Deciphering the Structure and Function of Nuclear Pores Using Single-Molecule Fluorescence Approaches.Journal of molecular biology · 2016Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Imaging single proteins or RNAs allows direct visualization of the inner workings of the cell. Typically, three-dimensional (3D) images are acquired by sequentially capturing a series of 2D sections. The time required to step through the sample often impedes imaging of large numbers of rapidly moving molecules. Here we applied multifocus microscopy (MFM) to instantaneously capture 3D single-molecule real-time images in live cells, visualizing cell nuclei at 10 volumes per second. We developed image analysis techniques to analyze messenger RNA (mRNA) diffusion in the entire volume of the nucleus. Combining MFM with precise registration between fluorescently labeled mRNA, nuclear pore complexes, and chromatin, we obtained globally optimal image alignment within 80-nm precision using transformation models. We show that β-actin mRNAs freely access the entire nucleus and fewer than 60% of mRNAs are more than 0.5 µm away from a nuclear pore, and we do so for the first time accounting for spatial inhomogeneity of nuclear organization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.