Evidence map›Paper›PMID 26008709›Full record

ArticleViruses2015

Characterization of novel transcripts in pseudorabies virus.

Dóra Tombácz, Zsolt Csabai, Péter Oláh, Zoltán Havelda, Donald Sharon, Michael Snyder, Zsolt Boldogkői

Open access · goldAbstract read
In one paragraph

Article in Viruses, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
7.2field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 46 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Dóra TombáczThese authors contributed equally to this work.. tombacz.dora@med.u-szeged.hu.
Zsolt CsabaiThese authors contributed equally to this work.. csabai.zsolt@med.u-szeged.hu.
Péter OláhThese authors contributed equally to this work.. olah.peter@med.u-szeged.hu.
Zoltán HaveldaDepartment of Medical Biology, Faculty of Medicine, University of Szeged, Somogyi B. u. 4., Szeged H-6720, Hungary. havelda@abc.hu.
Donald SharonAgricultural Biotechnology Center, Institute for Plant Biotechnology, Plant Developmental Biology Group, Szent-Györgyi A. u. 4, Gödöllő H-2100, Hungary. dsharon@stanford.edu.
Michael SnyderAgricultural Biotechnology Center, Institute for Plant Biotechnology, Plant Developmental Biology Group, Szent-Györgyi A. u. 4, Gödöllő H-2100, Hungary. mpsnyder@stanford.edu.
Zsolt BoldogkőiThese authors contributed equally to this work.. boldogkoi.zsolt@med.u-szeged.hu.
University of Szeged · HUStanford University · USAgricultural Biotechnology Institute · HU

Funding

Special EquipmentP50HG007735 · NHGRI · STANFORD UNIVERSITY · PI CHANG, HOWARD Y · 2014 to 2018
$15.2M
NHGRI NIH HHS 1P50HG007735-01NHGRI NIH HHS P50 HG007735
6 · The paper itself

Abstract

In this study we identified two 3'-coterminal RNA molecules in the pseudorabies virus. The highly abundant short transcript (CTO-S) proved to be encoded between the ul21 and ul22 genes in close vicinity of the replication origin (OriL) of the virus. The less abundant long RNA molecule (CTO-L) is a transcriptional readthrough product of the ul21 gene and overlaps OriL. These polyadenylated RNAs were characterized by ascertaining their nucleotide sequences with the Illumina HiScanSQ and Pacific Biosciences Real-Time (PacBio RSII) sequencing platforms and by analyzing their transcription kinetics through use of multi-time-point Real-Time RT-PCR and the PacBio RSII system. It emerged that transcription of the CTOs is fully dependent on the viral transactivator protein IE180 and CTO-S is not a microRNA precursor. We propose an interaction between the transcription and replication machineries at this genomic location, which might play an important role in the regulation of DNA synthesis.

Indexed as

Gene Expression ProfilingHerpesvirus 1, SuidHigh-Throughput Nucleotide SequencingReal-Time Polymerase Chain ReactionRNA, ViralTranscription, GeneticVirus ReplicationRNA, ViralDNA replicationherpesvirusnon-coding RNApseudorabies virusRNA sequencing

Identifiers

PMID26008709
PMCPMC4452928
OpenAlexW2157565191

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.