ArticleLab on a chip2015
High-throughput, motility-based sorter for microswimmers such as C. elegans.
Article in Lab on a chip, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 27 citations in OpenAlex.
- The utilization of small non-mammals in traumatic brain injury research: A systematic review.CNS neuroscience & therapeutics · 2021Pooled it
- GravSorter: a forward-genetics tool for studying gravity response inThe Analyst · 2026Article
- Deep Learning for Microfluidic-AssistedMicromachines · 2023Article
- A spiral microfluidic device for rapid sorting, trapping, and long-term live imaging ofMicrosystems & nanoengineering · 2023Article
- Microfluidic-AssistedCyborg and bionic systems (Washington, D.C.) · 2023Article
- The dihydropyrimidine dehydrogenase gene contributes to heritable differences in sleep in mice.Current biology : CB · 2021Article
- Caenorhabditis elegans exhibits positive gravitaxis.BMC biology · 2021Article
- Recent advances in understanding the genetics of sleep.F1000Research · 2020Review
- Inducing Mild Traumatic Brain Injury in C. elegans via Cavitation-Free Surface Acoustic Wave-Driven Ultrasonic Irradiation.Scientific reports · 2019Article
- Pneumatic stimulation of C. elegans mechanoreceptor neurons in a microfluidic trap.Lab on a chip · 2017Article
- Article
- Terrain following and applications: Caenorhabditis elegans swims along the floor using a bump and undulate strategy.Journal of the Royal Society, Interface · 2016Article
- Microfluidic Devices in Advanced Caenorhabditis elegans Research.Molecules (Basel, Switzerland) · 2016Review
- Geroscience approaches to increase healthspan and slow aging.F1000Research · 2016Review
- A hydrodynamic mechanism for attraction of undulatory microswimmers to surfaces (bordertaxis).Journal of the Royal Society, Interface · 2015Article
- Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Animal motility varies with genotype, disease, aging, and environmental conditions. In many studies, it is desirable to carry out high throughput motility-based sorting to isolate rare animals for, among other things, forward genetic screens to identify genetic pathways that regulate phenotypes of interest. Many commonly used screening processes are labor-intensive, lack sensitivity, and require extensive investigator training. Here, we describe a sensitive, high throughput, automated, motility-based method for sorting nematodes. Our method is implemented in a simple microfluidic device capable of sorting thousands of animals per hour per module, and is amenable to parallelism. The device successfully enriches for known C. elegans motility mutants. Furthermore, using this device, we isolate low-abundance mutants capable of suppressing the somnogenic effects of the flp-13 gene, which regulates C. elegans sleep. By performing genetic complementation tests, we demonstrate that our motility-based sorting device efficiently isolates mutants for the same gene identified by tedious visual inspection of behavior on an agar surface. Therefore, our motility-based sorter is capable of performing high throughput gene discovery approaches to investigate fundamental biological processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.