ArticleNucleic acids research2015
Disturbance-free rapid solution exchange for magnetic tweezers single-molecule studies.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Force-dependent structural dynamics of the giant nesprin-2.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Mechanically weak and highly dynamic state of mechanosensitive titin Ig domains induced by proline isomerization.Nature communications · 2025Article
- DNA-Based Molecular Clamp for Probing Protein Interactions and Structure under Force.ACS nano · 2024Article
- Single-molecule force spectroscopy reveals intra- and intermolecular interactions ofProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Multi-domain interaction mediated strength-building in human α-actinin dimers unveiled by direct single-molecule quantification.Nature communications · 2024Article
- Structural domain in the Titin N2B-us region binds to FHL2 in a force-activation dependent manner.Nature communications · 2024Article
- Article
- Insight into the biochemical mechanism of DNA helicases provided by bulk-phase and single-molecule assays.Methods (San Diego, Calif.) · 2022Review
- Dynamics and inhibition of MLL1 CXXC domain on DNA revealed by single-molecule quantification.Biophysical journal · 2021Article
- Talin mechanosensitivity is modulated by a direct interaction with cyclin-dependent kinase-1.The Journal of biological chemistry · 2021Article
- Single-molecule insight into stalled replication fork rescue in Escherichia coli.Nucleic acids research · 2021Review
- Modulating mechanical stability of heterodimerization between engineered orthogonal helical domains.Nature communications · 2020Article
- Article
- Phosphorylation Reduces the Mechanical Stability of the α-Catenin/ β-Catenin Complex.Angewandte Chemie (International ed. in English) · 2019Review
- Direct single-molecule quantification reveals unexpectedly high mechanical stability of vinculin-talin/α-catenin linkages.Science advances · 2019Article
- Dystrophin As a Molecular Shock Absorber.ACS nano · 2018Article
- Dissection of structural dynamics of chromatin fibers by single-molecule magnetic tweezers.Biophysics reports · 2018Article
- mDia1 senses both force and torque during F-actin filament polymerization.Nature communications · 2017Article
- Parallel triplex structure formed between stretched single-stranded DNA and homologous duplex DNA.Nucleic acids research · 2017Article
- Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers.Journal of visualized experiments : JoVE · 2017Article
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6 authors.
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Abstract
Single-molecule manipulation technologies have been extensively applied to studies of the structures and interactions of DNA and proteins. An important aspect of such studies is to obtain the dynamics of interactions; however the initial binding is often difficult to obtain due to large mechanical perturbation during solution introduction. Here, we report a simple disturbance-free rapid solution exchange method for magnetic tweezers single-molecule manipulation experiments, which is achieved by tethering the molecules inside microwells (typical dimensions-diameter (D): 40-50 μm, height (H): 100 μm; H:D∼2:1). Our simulations and experiments show that the flow speed can be reduced by several orders of magnitude near the bottom of the microwells from that in the flow chamber, effectively eliminating the flow disturbance to molecules tethered in the microwells. We demonstrate a wide scope of applications of this method by measuring the force dependent DNA structural transitions in response to solution condition change, and polymerization dynamics of RecA on ssDNA/SSB-coated ssDNA/dsDNA of various tether lengths under constant forces, as well as the dynamics of vinculin binding to α-catenin at a constant force (< 5 pN) applied to the α-catenin protein.
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