ArticleBMC musculoskeletal disorders2015
Temporal gene expression profiling of the rat knee joint capsule during immobilization-induced joint contractures.
Article in BMC musculoskeletal disorders, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 23 citations in OpenAlex.
- Histological analysis of collagen composition in the stifle joint capsule of dogs with congenital patellar luxation and cranial cruciate ligament rupture.Veterinary and animal science · 2026Article
- TECAR Therapy Associated with High-Intensity Laser Therapy (Hilt) and Manual Therapy in the Treatment of Muscle Disorders: A Literature Review on the Theorised Effects Supporting Their Use.Journal of clinical medicine · 2022Review
- Thermal and non-thermal effects of capacitive-resistive electric transfer application on different structures of the knee: a cadaveric study.Scientific reports · 2020Article
- Lumican promotes joint fibrosis through TGF-β signaling.FEBS open bio · 2020Article
- Mechanical adaptation of synoviocytes A and B to immobilization and remobilization: a study in the rat knee flexion model.Journal of molecular histology · 2020Article
- Hyaluronic acid-curcumin conjugate suppresses the fibrotic functions of myofibroblasts from contractive joint by the PTGER2 demethylation.Regenerative biomaterials · 2019Article
- Curcumin Inhibits Joint Contracture through PTEN Demethylation and Targeting PI3K/Akt/mTOR Pathway in Myofibroblasts from Human Joint Capsule.Evidence-based complementary and alternative medicine : eCAM · 2019Article
- C5a aggravates dysfunction of the articular cartilage and synovial fluid in rats with knee joint immobilization.Molecular medicine reports · 2018Article
- Intra-articular collagenase injection increases range of motion in a rat knee flexion contracture model.Drug design, development and therapy · 2018Article
- CORR InsightsClinical orthopaedics and related research · 2016Article
- mRNA Expression of Ovine Angiopoietin-like Protein 4 Gene in Adipose Tissues.Asian-Australasian journal of animal sciences · 2016Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundContractures of the knee joint cause disability and handicap. Recovering range of motion is recognized by arthritic patients as their preference for improved health outcome secondary only to pain management. Clinical and experimental studies provide evidence that the posterior knee capsule prevents the knee from achieving full extension. This study was undertaken to investigate the dynamic changes of the joint capsule transcriptome during the progression of knee joint contractures induced by immobilization. We performed a microarray analysis of genes expressed in the posterior knee joint capsule following induction of a flexion contracture by rigidly immobilizing the rat knee joint over a time-course of 16 weeks. Fold changes of expression values were measured and co-expressed genes were identified by clustering based on time-series analysis. Genes associated with immobilization were further analyzed to reveal pathways and biological significance and validated by immunohistochemistry on sagittal sections of knee joints.
resultsChanges in expression with a minimum of 1.5 fold changes were dominated by a decrease in expression for 7732 probe sets occurring at week 8 while the expression of 2251 probe sets increased. Clusters of genes with similar profiles of expression included a total of 162 genes displaying at least a 2 fold change compared to week 1. Functional analysis revealed ontology categories corresponding to triglyceride metabolism, extracellular matrix and muscle contraction. The altered expression of selected genes involved in the triglyceride biosynthesis pathway; AGPAT-9, and of the genes P4HB and HSP47, both involved in collagen synthesis, was confirmed by immunohistochemistry.
conclusionsGene expression in the knee joint capsule was sensitive to joint immobility and provided insights into molecular mechanisms relevant to the pathophysiology of knee flexion contractures. Capsule responses to immobilization was dynamic and characterized by modulation of at least three reaction pathways; down regulation of triglyceride biosynthesis, alteration of extracellular matrix degradation and muscle contraction gene expression. The posterior knee capsule may deploy tissue-specific patterns of mRNA regulatory responses to immobilization. The identification of altered expression of genes and biochemical pathways in the joint capsule provides potential targets for the therapy of knee flexion contractures.
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