ArticleInternational journal of environmental research and public health2015
Comparative Cytotoxicity Study of Silver Nanoparticles (AgNPs) in a Variety of Rainbow Trout Cell Lines (RTL-W1, RTH-149, RTG-2) and Primary Hepatocytes.
Article in International journal of environmental research and public health, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 71 citations in OpenAlex.
- Nanotechnology-Based Immunostimulants in Aquaculture: Mechanisms, Advances and Practical Applications.Veterinary medicine and science · 2026Review
- Structural, Adsorptive, and Antibacterial Properties of a Novel Silver (Diethyldithiocarbamate)-Decorated Reduced Graphene Oxide Nanocomposite for Sustainable Wastewater Treatment.Nanomaterials (Basel, Switzerland) · 2025Article
- Toxic Effects of Different Coating-Related Functionalized Nanoparticles on Aquatic Organisms.Toxics · 2024Article
- Review
- The Potentiating Effect of Graphene Oxide on the Arylhydrocarbon Receptor (AhR)-Cytochrome P4501A (Cyp1A) System Activated by Benzo(k)fluoranthene (BkF) in Rainbow Trout Cell Line.Nanomaterials (Basel, Switzerland) · 2023Article
- Green Synthesis of Silver Nanoparticles Using the Leaf Extract of the Medicinal Plant,Antibiotics (Basel, Switzerland) · 2023Article
- Toxicological Aspects, Safety Assessment, and Green Toxicology of Silver Nanoparticles (AgNPs)-Critical Review: State of the Art.International journal of molecular sciences · 2023Review
- AnRSC advances · 2022Article
- Wound Antiseptics and European Guidelines for Antiseptic Application in Wound Treatment.Pharmaceuticals (Basel, Switzerland) · 2021Review
- Cytotoxicity of Mycotoxins Frequently Present in Aquafeeds to the Fish Cell Line RTGill-W1.Toxins · 2021Article
- Concentration-dependent toxicogenomic changes of silver nanoparticles in hepatocyte-like cells derived from human induced pluripotent stem cells.Cell biology and toxicology · 2021Article
- Endocytosis, intracellular fate, accumulation, and agglomeration of titanium dioxide (TiOEnvironmental science and pollution research international · 2019Article
- Antimicrobial activity of nano-sized silver colloids stabilized by nitrogen-containing polymers: the key influence of the polymer capping.RSC advances · 2018Article
- Cytotoxicity of silver and copper nanoparticles on rainbow trout (Oncorhynchus mykiss) hepatocytes.Environmental science and pollution research international · 2018Article
- In vitro or not in vitro: a short journey through a long history.Environmental sciences Europe · 2018Review
- Interaction of silver nanoparticles with algae and fish cells: a side by side comparison.Journal of nanobiotechnology · 2017Article
- Assessing the bioactivity of crystalline silica in heated high-temperature insulation wools.Inhalation toxicologyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Among all classes of nanomaterials, silver nanoparticles (AgNPs) have potentially an important ecotoxicological impact, especially in freshwater environments. Fish are particularly susceptible to the toxic effects of silver ions and, with knowledge gaps regarding the contribution of dissolution and unique particle effects to AgNP toxicity, they represent a group of vulnerable organisms. Using cell lines (RTL-W1, RTH-149, RTG-2) and primary hepatocytes of rainbow trout (Oncorhynchus mykiss) as in vitro test systems, we assessed the cytotoxicity of the representative AgNP, NM-300K, and AgNO3 as an Ag+ ion source. Lack of AgNP interference with the cytotoxicity assays (AlamarBlue, CFDA-AM, NRU assay) and their simultaneous application point to the compatibility and usefulness of such a battery of assays. The RTH-149 and RTL-W1 liver cell lines exhibited similar sensitivity as primary hepatocytes towards AgNP toxicity. Leibovitz's L-15 culture medium composition (high amino acid content) had an important influence on the behaviour and toxicity of AgNPs towards the RTL-W1 cell line. The obtained results demonstrate that, with careful consideration, such an in vitro approach can provide valuable toxicological data to be used in an integrated testing strategy for NM-300K risk assessment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.