ArticleFrontiers in molecular neuroscience2015
Intracellular calcium dynamics in cortical microglia responding to focal laser injury in the PC::G5-tdT reporter mouse.
Article in Frontiers in molecular neuroscience, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed.
- Calcium signaling in microglial immune functions: current understanding and implications for disease.Current opinion in immunology · 2026Review
- Microglia respond to and induce anxiety and grooming in mice using calcium signaling.Molecular psychiatry · 2026Article
- Calcium Signaling in Macrophages During a Wound Response In Vivo.International journal of molecular sciences · 2026Article
- Review
- Article
- TSC-associated microglial hyperactivity: enhanced calcium signaling, metabolism, and phagocytosis.Acta neuropathologica · 2026Article
- Investigating the Role of Cortical Microglia in a Mouse Model of Viral Infection-Induced Seizures.eNeuro · 2026Article
- Sub-50-femtosecond gain-managed amplified pulses enhance nonlinear ablation efficiency.Biomedical optics express · 2026Article
- Modulation of Ryanodine Receptors on Microglial Ramification, Migration, and Phagocytosis in an Alzheimer's Disease Mouse Model.Neuroscience bulletin · 2025Article
- Morphotype-specific calcium signaling in human microglia.Journal of neuroinflammation · 2024Article
- Microglial P2YNeuron · 2024Article
- Optogenetic stimulation of mouse Hoxb8 microglia in specific regions of the brain induces anxiety, grooming, or both.Molecular psychiatry · 2024Article
- Reactive microglia fail to respond to environmental damage signals in a viral-induced mouse model of temporal lobe epilepsy.bioRxiv : the preprint server for biology · 2024Article
- Amyloid plaques and normal ageing have differential effects on microglial CaPflugers Archiv : European journal of physiology · 2024Article
- Microglia sense astrocyte dysfunction and prevent disease progression in an Alexander disease model.Brain : a journal of neurology · 2024Article
- Progress in Structural and Functional In Vivo Imaging of Microglia and Their Application in Health and Disease.Advances in neurobiology · 2024Review
- Article
- Gap Junctions and Connexins in Microglia-Related Oxidative Stress and Neuroinflammation: Perspectives for Drug Discovery.Biomolecules · 2023Review
- Chemogenetic and Optogenetic Manipulations of Microglia in Chronic Pain.Neuroscience bulletin · 2023Review
- Preclinical models of middle cerebral artery occlusion: new imaging approaches to a classic technique.Frontiers in neurology · 2023Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Microglia, the resident immune cells of the brain parenchyma, are highly responsive to tissue injury. Following cell damage, microglial processes redirect their motility from randomly scouting the extracellular space to specifically reaching toward the compromised tissue. While the cell morphology aspects of this defense mechanism have been characterized, the intracellular events underlying these responses remain largely unknown. Specifically, the role of intracellular Ca(2+) dynamics has not been systematically investigated in acutely activated microglia due to technical difficulty. Here we used live two-photon imaging of the mouse cortex ubiquitously expressing the genetically encoded Ca(2+) indicator GCaMP5G and fluorescent marker tdTomato in central nervous system microglia. We found that spontaneous Ca(2+) transients in microglial somas and processes were generally low (only 4% of all microglia showing transients within 20 min), but baseline activity increased about 8-fold when the animals were treated with LPS 12 h before imaging. When challenged with focal laser injury, an additional surge in Ca(2+) activity was observed in the somas and protruding processes. Notably, coherent and simultaneous Ca(2+) rises in multiple microglial cells were occasionally detected in LPS-treated animals. We show that Ca(2+) transients were pre-dominantly mediated via purinergic receptors. This work demonstrates the usefulness of genetically encoded Ca(2+) indicators for investigation of microglial physiology.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.