Evidence map›Paper›PMID 26004631›Full record

ArticleStem cell reports2015

hESC Differentiation toward an Autonomic Neuronal Cell Fate Depends on Distinct Cues from the Co-Patterning Vasculature.

Lisette M Acevedo, Jeffrey N Lindquist, Breda M Walsh, Peik Sia, Flavio Cimadamore, Connie Chen, Martin Denzel, Cameron D Pernia, Barbara Ranscht, Alexey Terskikh and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Vascularized Organoids: A More Complete Model.International journal of stem cells · 2021
    Review
  3. Article
  4. Article
  5. Article
  6. Review
  7. Blood vessel crosstalk during organogenesis-focus on pancreas and endothelial cells.Wiley interdisciplinary reviews. Developmental biology · 2016
    Review
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Lisette M AcevedoMoores Cancer Center, University of California, San Diego, La Jolla, CA, 92093, USA; Sanford Burnham Medical Research Institute, La Jolla, CA 92037, USA; Department of Pathology, University of California, San Diego, La Jolla, CA 92093, USA.
Jeffrey N LindquistMoores Cancer Center, University of California, San Diego, La Jolla, CA, 92093, USA; Sanford Burnham Medical Research Institute, La Jolla, CA 92037, USA.
Breda M WalshMoores Cancer Center, University of California, San Diego, La Jolla, CA, 92093, USA.
Peik SiaMoores Cancer Center, University of California, San Diego, La Jolla, CA, 92093, USA.
Flavio CimadamoreSanford Burnham Medical Research Institute, La Jolla, CA 92037, USA.
Connie ChenSanford Burnham Medical Research Institute, La Jolla, CA 92037, USA.
Martin DenzelSanford Burnham Medical Research Institute, La Jolla, CA 92037, USA.
Cameron D PerniaSanford Burnham Medical Research Institute, La Jolla, CA 92037, USA; Sanford Consortium for Regenerative Medicine, La Jolla, CA 92037, USA.
Barbara RanschtSanford Burnham Medical Research Institute, La Jolla, CA 92037, USA.
Alexey TerskikhSanford Burnham Medical Research Institute, La Jolla, CA 92037, USA.
Evan Y SnyderSanford Burnham Medical Research Institute, La Jolla, CA 92037, USA; Sanford Consortium for Regenerative Medicine, La Jolla, CA 92037, USA; Department of Pediatrics, University of California, San Diego, La Jolla, CA 92093, USA. Electronic address: esnyder@sanfordburnham.org.
David A ChereshMoores Cancer Center, University of California, San Diego, La Jolla, CA, 92093, USA; Sanford Consortium for Regenerative Medicine, La Jolla, CA 92037, USA; Department of Pathology, University of California, San Diego, La Jolla, CA 92093, USA. Electronic address: dcheresh@ucsd.edu.

Funding

The Stem Cell Center at The Burnham InstituteP20GM075059 · NIGMS · SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE · PI SNYDER, EVAN YALE · 2005 to 2007
$3.0M
Effects of Sema3A-Nrp1 signaling on tumor cell extravastion and metastasisK01CA148897 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ACEVEDO, LISETTE MARIE · 2010 to 2013
$432k
NCI NIH HHS K01 CA148897NCI NIH HHS NIH-K01CA148897NIGMS NIH HHS NIH-P20GM075059NIGMS NIH HHS P20 GM075059
6 · The paper itself

Abstract

To gain insight into the cellular and molecular cues that promote neurovascular co-patterning at the earliest stages of human embryogenesis, we developed a human embryonic stem cell model to mimic the developing epiblast. Contact of ectoderm-derived neural cells with mesoderm-derived vasculature is initiated via the neural crest (NC), not the neural tube (NT). Neurovascular co-patterning then ensues with specification of NC toward an autonomic fate requiring vascular endothelial cell (EC)-secreted nitric oxide (NO) and direct contact with vascular smooth muscle cells (VSMCs) via T-cadherin-mediated homotypic interactions. Once a neurovascular template has been established, NT-derived central neurons then align themselves with the vasculature. Our findings reveal that, in early human development, the autonomic nervous system forms in response to distinct molecular cues from VSMCs and ECs, providing a model for how other developing lineages might coordinate their co-patterning.

Indexed as

AnimalsBlood VesselsCadherinsCell Culture TechniquesCell DifferentiationCell LineageCells, CulturedCoculture TechniquesEctodermEndothelium, VascularHuman Embryonic Stem CellsHumansImmunohistochemistryMaleMesodermMiceCadherinsH-cadherinNitric OxideNitric Oxide DonorsNitric Oxide Synthase Type IIIPeripherinsPRPH protein, humanRNA, Small InterferingTubulin

Identifiers

PMID26004631
PMCPMC4471822

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.